Mu Xupeng, Li Jing, Yan Shaohua, Zhang Hongmei, Zhang Wenjing, Zhang Fuqiang, Jiang Jinlan
Department of Central Laboratory, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130033, P. R. China.
Department of Biological Engineering, College of Pharmacy, Jilin University, 1163 Xinmin Street, Changchun 130021, P. R. China.
ACS Biomater Sci Eng. 2018 Nov 12;4(11):3895-3905. doi: 10.1021/acsbiomaterials.8b00858. Epub 2018 Oct 2.
Biomimetic cell membrane coated nanoparticles (NPs) with desirable features have been extensively applied for various personalized biomedicine. However, there have not been relative explorations by employing the membrane nanocomplexes for small interfering RNA (siRNA) delivery. Herein, FeO@PDA NPs with good photothermal capability were applied for efficient siRNA loading and delivery, which were then coated by mesenchymal stem cells (MSCs) to form a membrane. The data showed that MSCs membrane coated FeO@PDA-siRNA NPs (FeO@PDA-siRNA@MSCs) maintained the photothermal functionality and the capability of magnetic resonance imaging inherited from FeO@PDA. The synthesized nanocomplexes exhibited excellent abilities in the delivery of siRNA into DU145 cells. Furthermore, FeO@PDA-siRNA@MSCs NPs delivering siRNA against Plk1 gene could inhibit the expression of endogenous Plk1 gene and cause obvious apoptosis in DU145 cells. The synergistic combination of photothermal treatment and gene silencing showed obvious antitumor efficacy in a DU145 xenograft mice model. On the basis of preliminary and studies, FeO@PDA-siRNA@MSCs NPs hold considerable promise as a carrier for gene and photothermal therapy.
具有理想特性的仿生细胞膜包覆纳米颗粒（NPs）已被广泛应用于各种个性化生物医学领域。然而，利用膜纳米复合物进行小干扰RNA（siRNA）递送的相关探索尚未见报道。在此，将具有良好光热性能的FeO@PDA NPs用于高效的siRNA负载和递送，然后用间充质干细胞（MSCs）包覆形成一层膜。数据表明，MSCs膜包覆的FeO@PDA-siRNA NPs（FeO@PDA-siRNA@MSCs）保留了源自FeO@PDA的光热功能和磁共振成像能力。合成的纳米复合物在将siRNA递送至DU145细胞方面表现出优异的能力。此外，递送针对Plk1基因的siRNA的FeO@PDA-siRNA@MSCs NPs可抑制内源性Plk1基因的表达，并在DU145细胞中引起明显的凋亡。光热处理和基因沉默的协同组合在DU145异种移植小鼠模型中显示出明显的抗肿瘤效果。基于初步和研究，FeO@PDA-siRNA@MSCs NPs作为基因和光热治疗的载体具有很大的前景。
