Trinkaus-Randall V, Capecchi J, Newton A, Vadasz A, Leibowitz H, Franzblau C
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Invest Ophthalmol Vis Sci. 1988 Mar;29(3):393-400.
Based on the results of in vitro and in vivo experiments, we have determined that the optimal material for the central transparent portion of a perforating keratoprosthesis is a polyvinyl alcohol copolymer hydrogel. The material supports the maintenance and growth of corneal epithelium in vitro as shown by population doublings and transmission electron microscopy. Discs preseeded with epithelial cells were cultured in vitro and transplanted into rabbit corneas. The proliferation of these cells in vivo was demonstrated using 3H-thymidine. Other experiments showed that the preseeded cells not only migrated from the central disc onto the peripheral rim of the host cornea but also that host peripheral epithelial cells migrated onto the anterior surface of the disc. The experiments described in this paper demonstrate that corneal epithelial cells preseeded onto hydrogel discs and transplanted into rabbit corneas remain adherent and are capable of proliferating.
基于体外和体内实验结果，我们已确定，穿透性角膜移植假体中央透明部分的最佳材料是聚乙烯醇共聚物水凝胶。如群体倍增和透射电子显微镜所示，该材料在体外支持角膜上皮的维持和生长。预先接种上皮细胞的圆盘在体外培养并移植到兔角膜中。使用³H-胸腺嘧啶核苷证明了这些细胞在体内的增殖。其他实验表明，预先接种的细胞不仅从中央圆盘迁移到宿主角膜的周边边缘，而且宿主周边上皮细胞也迁移到圆盘的前表面。本文所述实验表明，预先接种到水凝胶圆盘上并移植到兔角膜中的角膜上皮细胞保持附着并能够增殖。
