Song Menglin, Yang Mo, Hao Jianhua
Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon 999077, Hong Kong, P.R. China.
Department of Biomedical Engineering, The Hong Kong Polytechnic University, Kowloon 999077, Hong Kong, P.R. China.
Cell Rep Phys Sci. 2021 Jan 20;2(1):100288. doi: 10.1016/j.xcrp.2020.100288. Epub 2021 Jan 6.
The novel coronavirus pandemic is sweeping the world and causing global crises. The lack of effective methods of early diagnosis and accurate detection may result in severe infection as well as mortality. Therefore, it is urgently required that rapid, selective, and accurate techniques for detecting pathogenic viruses are developed. Nanotechnology-based biosensors are finding many applications in biological detection, which may address these issues and realize direct detection of molecular targets in real time. Among various nanoplatforms, optical nanobiosensors have aroused much interest due to their inherent advantages of high sensitivity and direct readout. In this review, a summary of recent progress on the optical biosensors based on nanotechnology for pathogenic virus detection is provided, with focus on quantum dots (QDs), upconversion nanoparticles (UCNPs), noble metal nanoparticles, and organic fluorescent molecules-based nanoprobes and chemiluminescence assays. These representative studies demonstrate appealing performance as biosensors and hold great promise for clinical diagnosis.
新型冠状病毒大流行正在席卷全球并引发全球危机。缺乏有效的早期诊断和准确检测方法可能导致严重感染以及死亡。因此，迫切需要开发快速、选择性和准确的病原病毒检测技术。基于纳米技术的生物传感器在生物检测中有着诸多应用，这可能解决这些问题并实现分子靶点的实时直接检测。在各种纳米平台中，光学纳米生物传感器因其高灵敏度和直接读出的固有优势而引起了广泛关注。在本综述中，总结了基于纳米技术的用于病原病毒检测的光学生物传感器的最新进展，重点关注量子点（QDs）、上转换纳米颗粒（UCNPs）、贵金属纳米颗粒以及基于有机荧光分子的纳米探针和化学发光分析。这些代表性研究展示了作为生物传感器的诱人性能，并在临床诊断方面具有巨大潜力。
