Department of General Surgery, Zhujiang Hospital, Southern Medical University/The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.
Department of General Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):83-95. doi: 10.1080/21691401.2021.1871620.
Cancer-targeted drug delivery systems based on nanoparticles (NPs) have been considered promising therapies. In this study, we developed a pH-responsive smart NPs drug delivery system using silk fibroin (SF), selenium nanoparticles (Se NPs), fingolimod (FTY720), and heptapeptide (T7). The prepared FTY720@T7-SF-Se NPs were spheres with an average diameter of 120 nm, which would contribute to the enhanced permeability and retention effects in tumour regions. The encapsulation efficiency (EE) of the FTY720@T7-SF-Se NPs was 71.95 ± 3.81%. The release of FTY720 from the nanocarriers was pH-dependent, and the release of FTY720 was accelerated in an acidic environment. Both and studies showed that FTY720@T7-SF-Se NPs had an enhanced cellular uptake selectivity and antitumor activity for thyroid cancer. The bio-distribution study further demonstrated that FTY720@T7-SF-Se NPs could effectively accumulate in the tumour region, thereby enhancing the ability to kill cancer cells . In addition, studies of histology and immunohistochemistry showed that FTY720@T7-SF-Se NPs had low toxicity to the major organs of tumour-bearing mice, indicating the prepared NPs has good biocompatibility . These results suggest that the tumour-targeted NPs delivery system (FTY720@T7-SF-Se NPs) has great potential as a new tool for thyroid cancer therapy.
基于纳米粒子（NPs）的癌症靶向药物输送系统被认为是很有前途的治疗方法。在这项研究中，我们使用丝素蛋白（SF）、硒纳米粒子（Se NPs）、芬戈莫德（FTY720）和七肽（T7）开发了一种 pH 响应型智能 NPs 药物输送系统。制备的 FTY720@T7-SF-Se NPs 为球体，平均直径为 120nm，有助于增强肿瘤区域的通透性和保留效应。FTY720@T7-SF-Se NPs 的包封效率（EE）为 71.95±3.81%。FTY720 从纳米载体中的释放是 pH 依赖性的，在酸性环境中释放加速。和 研究均表明，FTY720@T7-SF-Se NPs 对甲状腺癌细胞具有增强的细胞摄取选择性和抗肿瘤活性。生物分布研究 进一步表明，FTY720@T7-SF-Se NPs 可以有效地在肿瘤区域积聚，从而增强杀死癌细胞的能力 。此外，对荷瘤小鼠主要器官的组织学和免疫组织化学研究表明，FTY720@T7-SF-Se NPs 对其毒性较低，表明所制备的 NPs 具有良好的生物相容性 。这些结果表明，肿瘤靶向 NPs 输送系统（FTY720@T7-SF-Se NPs）具有作为甲状腺癌治疗新工具的巨大潜力。
