Strehmel Christine, Perez-Hernandez Heidi, Zhang Zhenfang, Löbus Axel, Lasagni Andrés F, Lensen Marga C
Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 124, D-10623 Berlin, Germany.
Institute of Manufacturing Technology, Technische Universität Dresden, George-Bähr-Straße 3c, D-01062 Dresden, Germany.
ACS Biomater Sci Eng. 2015 Sep 14;1(9):747-752. doi: 10.1021/ab5001657. Epub 2015 Aug 5.
In this study, a mask-less laser-assisted patterning method is used to fabricate well-defined cell-adhesive microdomains delimited by protein-repellent poly(ethylene glycol) (PEG) microstructures prepared from multiarm (8-PEG) macromonomers. The response of murine fibroblasts (L-929) toward these microdomains is investigated, revealing effective cell confinement within the cell-adhesive areas surrounded by nonadhesive 8-PEG microstructures. Moreover, the spatial positioning of cells in microdomains of various sizes and geometries is analyzed, indicating control of cell density, size, and elongated cell shape induced by the size of the microdomains and the geometric confinement.
在本研究中，一种无掩膜激光辅助图案化方法被用于制造由多臂（8-聚乙二醇）大分子单体制备的蛋白质排斥性聚（乙二醇）（PEG）微结构所界定的明确的细胞粘附微区。研究了小鼠成纤维细胞（L-929）对这些微区的反应，揭示了细胞在由非粘附性8-PEG微结构包围的细胞粘附区域内的有效限制。此外，分析了细胞在各种大小和几何形状的微区内的空间定位，表明微区大小和几何限制对细胞密度、大小和伸长细胞形状具有控制作用。
