卵巢癌中多种生物标志物的检测与分析：在诊断、治疗及预后评估中的临床意义

Detection and analysis of multiple biomarkers in ovarian cancer: clinical significance in diagnosis, treatment, and prognosis evaluation.

作者信息

Ji Rui, Li Yong, He Chenyun, Zhu Xinghua, He Aiqin, Lu Yunyan

机构信息

Department of Gynecology, Affiliated Cancer Hospital of Nantong University, Nantong, China.

Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong, China.

出版信息

Gland Surg. 2020 Dec;9(6):2175-2186. doi: 10.21037/gs-20-811.

DOI:10.21037/gs-20-811

PMID:33447568

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7804549/

Abstract

BACKGROUND

The purpose of this study was to explore the clinical significance of CA125, CK7, CK20, ER, PR, C-erbb2, and P-gp in ovarian cancer.

METHODS

Ovarian cancer patients were recruited from Nantong Cancer Hospital between March 2006 and July 2011. The expressions of CA125, CK7, CK20, ER, PR, C-erbb2, and P-gp were determined by immunohistochemistry (IHC).The chi-square test (χ) was used to analyze the correlation between each index and the clinical characteristics of the patients. The patients were followed up to record the cancer recurrence time. The Kaplan-Meier method was used to map the cumulative recurrence-free survival (RFS) rate, and COX regression analysis was established for multivariate analysis.

RESULTS

The results of IHC showed that the positive expression rates of CA125, CK7, ER, C-erbb2, and P-gp in malignant ovarian cancer tissues were significantly higher than those in benign ovarian cancer tissues. CA125 expression in malignant ovarian cancer was significantly correlated with the age of patients and the Federation of International Gynecology and Obstetrics (FIGO) stage. CK7 expression in malignant ovarian cancer was significantly correlated with the age, tissue differentiation, and number of residual lesions. CK20 expression in malignant ovarian cancer was significantly correlated with the age and tissue differentiation of the patients. ER expression in malignant ovarian cancer was significantly correlated with the age of patients and FIGO stage. PR expression in malignant ovarian cancer was significantly correlated with the age of the patients. C-erbb2 expression in malignant ovarian cancer was significantly correlated with the age of the patients. P-gp expression in malignant ovarian cancer was significantly correlated with the patient age, pathological type, and tissue differentiation. The expression of CA125, CK7, CK20, C-erbb2, and P-gp had significant effects on the prognosis of patients with ovarian cancer. The COX regression analysis showed that was an independent risk factor for ovarian cancer.

CONCLUSIONS

In malignant ovarian cancer tissues, CA125, CK7, CK20, ER, PR, C-erbb2, and P-gp are over-expressed. The expression of P-gp is an independent risk factor for ovarian cancer, and it can be an important target for the treatment of malignant ovarian cancer.

摘要

背景

本研究旨在探讨CA125、细胞角蛋白7（CK7）、细胞角蛋白20（CK20）、雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2（C-erbb2）和P-糖蛋白（P-gp）在卵巢癌中的临床意义。

方法

选取2006年3月至2011年7月在南通肿瘤医院就诊的卵巢癌患者。采用免疫组织化学法检测CA125、CK7、CK20、ER、PR、C-erbb2和P-gp的表达。采用卡方检验分析各指标与患者临床特征的相关性。对患者进行随访，记录癌症复发时间。采用Kaplan-Meier法绘制累积无复发生存率（RFS）曲线，并进行COX回归分析以进行多因素分析。

结果

免疫组织化学结果显示，恶性卵巢癌组织中CA125、CK7、ER、C-erbb2和P-gp的阳性表达率显著高于良性卵巢癌组织。恶性卵巢癌中CA125的表达与患者年龄及国际妇产科联盟（FIGO）分期显著相关。恶性卵巢癌中CK7的表达与年龄、组织分化程度及残留病灶数量显著相关。恶性卵巢癌中CK20的表达与患者年龄及组织分化程度显著相关。恶性卵巢癌中ER的表达与患者年龄及FIGO分期显著相关。恶性卵巢癌中PR的表达与患者年龄显著相关。恶性卵巢癌中C-erbb2的表达与患者年龄显著相关。恶性卵巢癌中P-gp的表达与患者年龄、病理类型及组织分化程度显著相关。CA125、CK7、CK20、C-erbb2和P-gp的表达对卵巢癌患者的预后有显著影响。COX回归分析显示，[此处原文缺失具体内容]是卵巢癌的独立危险因素。