Cotty P J
Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179.
Appl Environ Microbiol. 1988 Jan;54(1):274-6. doi: 10.1128/aem.54.1.274-276.1988.
Aflatoxin concentrations in agar media were estimated with a direct technique that quantifies the fluorescence of agar containing aflatoxins. Tubes containing 5 ml of an agar medium inoculated with spores of aflatoxin-producing Aspergillus isolates were incubated for 3 days at 30 degrees C and set in a carriage specifically designed to carry culture tubes in a scanning densitometer. Fluorescence (450 nm and above) was elicited in the agar by UV light (365 nm) and photometrically measured. Agar fluorescence directly correlated (r2 = 0.89 +/- 0.05, P less than 0.001) with the concentration of aflatoxin within the range 0 to 18.7 micrograms/g. The lowest aflatoxin concentration detected was 50 ng/g. The technique successfully differentiated the aflatoxigenic potentials of Aspergillus isolates.
采用一种直接技术来估算琼脂培养基中的黄曲霉毒素浓度，该技术可对含有黄曲霉毒素的琼脂的荧光进行定量。将装有5毫升接种了产黄曲霉毒素曲霉菌株孢子的琼脂培养基的试管在30℃下培养3天，然后放入专门设计用于在扫描密度计中放置培养管的托架中。通过紫外光（365nm）激发琼脂中的荧光（450nm及以上）并进行光度测量。在0至18.7微克/克的范围内，琼脂荧光与黄曲霉毒素浓度直接相关（r2 = 0.89±0.05，P<0.001）。检测到的最低黄曲霉毒素浓度为50纳克/克。该技术成功区分了曲霉菌株的产黄曲霉毒素潜力。
