NanoBioMedical Centre, Adam Mickiewicz University in Poznań, Wszechnicy Piastowskiej 3, PL-61614 Poznań, Poland.
Faculty of Physics, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 2, PL-61614 Poznań, Poland.
Int J Mol Sci. 2021 Jan 13;22(2):738. doi: 10.3390/ijms22020738.
The development of multifunctional drug delivery systems combining two or more nanoparticle-mediated therapies for efficient cancer treatment is highly desired. To face this challenge, a photothermally active polydopamine (PDA) nanoparticle-based platform was designed for the loading of chemotherapeutic drug and targeting of cancer cells. PDA spheres were first functionalized with polyamidoamine (PAMAM) dendrimers followed by the conjugation with polyethylene glycol (PEG) moieties and folic acid (FA) targeting ligand. The anticancer drug doxorubicin (DOX) was then absorbed on the particle surface. We performed the physico-chemical characterization of this versatile material and we assessed further its possible application in chemo- and photothermal therapy using liver cancer cell model. These nanoparticles exhibited high near-infrared photothermal conversion efficacy and allowed for loading of the drug, which upon release in specifically targeted cancer cells suppressed their growth. Using cell proliferation, membrane damage, apoptosis, and oxidative stress assays we demonstrated high performance of this nanosystem in cancer cell death induction, providing a novel promising approach for cancer therapy.
为了高效治疗癌症，人们非常希望开发将两种或多种基于纳米颗粒的治疗方法结合起来的多功能药物输送系统。为了应对这一挑战，设计了一种基于光热活性聚多巴胺（PDA）纳米颗粒的平台，用于装载化疗药物和靶向癌细胞。PDA 球首先用聚酰胺-胺（PAMAM）树枝状大分子进行功能化，然后与聚乙二醇（PEG）部分和叶酸（FA）靶向配体缀合。然后将抗癌药物阿霉素（DOX）吸附在颗粒表面。我们对这种多功能材料进行了物理化学特性分析，并进一步评估了其在使用肝癌细胞模型的化学和光热治疗中的可能应用。这些纳米粒子表现出高近红外光热转换效率，并允许装载药物，药物在特定靶向癌细胞中释放后抑制其生长。通过细胞增殖、膜损伤、细胞凋亡和氧化应激测定，我们证明了该纳米系统在诱导癌细胞死亡方面的高性能，为癌症治疗提供了一种新的有前途的方法。
