Liu Jun, Yang Fan, Feng Miao, Wang Yanxing, Peng Xiangrong, Lv Ruichan
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5051-5059. doi: 10.1021/acsbiomaterials.9b01112. Epub 2019 Aug 28.
In this research, we design the integration of Au/Ag nanocages with upconversion nanoparticles (UCNPs) as the theranostic agent under single 808 nm with enhanced imaging-guided photodynamic therapy (PDT) and photothermal therapy (PTT) properties. Different with the conventional theranostic agent, Au/Ag@UCNPs can emit higher blue emission under 808 nm laser and generate higher reactive oxygen species than that of Au@UCNPs due to higher crossed absorbance between the nanocages and UCNPs. Furthermore, the temperature change of Au/Ag@UCNPs (9.7 °C) is much higher than that of phosphate-buffered saline solution (0.6 °C) under 808 nm laser, indicating there is a low side effect to normal cells when Au/Ag@UCNPs are utilized as the photoactive agent. Finally, the in vitro and in vivo experiments show that the tumor is almost ablated totally due to high synergistic PDT and PTT effects of Au/Ag@UCNPs, revealing it could be potentially applied in the clinical theranostic field.
在本研究中，我们设计将金/银纳米笼与上转换纳米粒子（UCNPs）集成作为治疗诊断剂，在单一808 nm光下具有增强的成像引导光动力疗法（PDT）和光热疗法（PTT）性能。与传统治疗诊断剂不同，由于纳米笼与UCNPs之间更高的交叉吸收率，Au/Ag@UCNPs在808 nm激光下可发出更高强度的蓝光发射，并比Au@UCNPs产生更多的活性氧。此外，在808 nm激光下，Au/Ag@UCNPs的温度变化（9.7℃）远高于磷酸盐缓冲盐溶液的温度变化（0.6℃），这表明当Au/Ag@UCNPs用作光活性剂时对正常细胞的副作用较小。最后，体外和体内实验表明，由于Au/Ag@UCNPs具有高度协同的PDT和PTT效应，肿瘤几乎被完全消融，这表明它有可能应用于临床治疗诊断领域。
