Arrondo Nair Vilas, Gomes-Dos-Santos André, Román Marcote Esther, Pérez Montse, Froufe Elsa, Castro L Filipe C
AQUACOV, Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Vigo, Spain.
Faculty of Biology, UVIGO, PhD Program "Marine Science, Technology and Management" (DoMAR), University of Vigo, Vigo, Spain.
Mitochondrial DNA B Resour. 2020 Jul 15;5(3):2850-2852. doi: 10.1080/23802359.2020.1790325.
Species of the Sternoptychidae teleost family display an impressive morphology, including their extreme reduced size. Here, we report the first mitochondrial genome of the diaphanous hatchet fish . By using short-read sequencing Illumina HiSeq, we generated two mitochondrial contigs which were later physically assembled by PCR. The mitochondrial genome of S. diaphana was 17,224 bp in length (excluding the control region) and is composed of 13 PCGs and 2 ribosomal RNA genes. Strikingly, we could not identify the tRNA-Phe and two copies of tRNA-Met were differently positioned. Additionally, the mitogenome displays a completely new gene rearrangement among vertebrates. We expect that the study presented here will pave the way for further molecular studies with this underrepresented group of illusive teleost fish.
星光鱼科硬骨鱼的物种呈现出令人印象深刻的形态,包括它们极小的体型。在此,我们报告了透明斧鱼的首个线粒体基因组。通过使用Illumina HiSeq短读长测序技术,我们生成了两个线粒体重叠群,随后通过聚合酶链式反应(PCR)进行了物理组装。透明斧鱼的线粒体基因组长度为17,224碱基对(不包括控制区),由13个蛋白质编码基因(PCGs)和2个核糖体RNA基因组成。引人注目的是,我们未能鉴定出苯丙氨酸转运RNA(tRNA-Phe),并且两个甲硫氨酸转运RNA(tRNA-Met)的拷贝位置不同。此外,该线粒体基因组在脊椎动物中展示了一种全新的基因重排。我们期望本文所呈现的研究将为对这种研究较少的神秘硬骨鱼群体进行进一步的分子研究铺平道路。