Duan Yuange, Dou Shengqian, Porath Hagit T, Huang Jiaxing, Eisenberg Eli, Lu Jian
State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China.
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
iScience. 2021 Jan 5;24(1):101983. doi: 10.1016/j.isci.2020.101983. eCollection 2021 Jan 22.
Social insects exhibit extensive phenotypic diversities among the genetically similar individuals, suggesting a role for the epigenetic regulations beyond the genome level. The ADAR-mediated adenosine-to-inosine (A-to-I) RNA editing, an evolutionarily conserved mechanism, facilitates adaptive evolution by expanding proteomic diversities. Here, we characterize the A-to-I RNA editome of honeybees (), identifying 407 high-confidence A-to-I editing sites. Editing is most abundant in the heads and shows signatures for positive selection. Editing behavior differs between foragers and nurses, suggesting a role for editing in caste differentiation. Although only five sites are conserved between bees and flies, an unexpectedly large number of genes exhibit editing in both species, albeit at different locations, including the nonsynonymous auto-editing of . This convergent evolution, where the same target genes independently acquire recoding events in distant diverged clades, together with the signals of adaptation observed in honeybees alone, further supports the notion of recoding being adaptive.
社会性昆虫在基因相似的个体间表现出广泛的表型多样性，这表明表观遗传调控在基因组水平之外发挥了作用。ADAR介导的腺苷到肌苷（A-to-I）RNA编辑是一种进化上保守的机制，通过扩展蛋白质组多样性促进适应性进化。在这里，我们对蜜蜂的A-to-I RNA编辑组进行了表征，鉴定出407个高可信度的A-to-I编辑位点。编辑在头部最为丰富，并显示出正选择的特征。觅食者和护理者的编辑行为有所不同，这表明编辑在 caste 分化中发挥了作用。虽然蜜蜂和苍蝇之间仅5个位点保守，但出乎意料的是，大量基因在两个物种中均表现出编辑，尽管位置不同，包括[具体基因]的非同义自编辑。这种趋同进化，即相同的靶基因在远缘分化的类群中独立获得重新编码事件，以及仅在蜜蜂中观察到的适应性信号，进一步支持了重新编码具有适应性的观点。
