Kuznetsova Vera, Osipova Viktoria, Tkach Anton, Miropoltsev Maksim, Kurshanov Danil, Sokolova Anastasiia, Cherevkov Sergei, Zakharov Viktor, Fedorov Anatoly, Baranov Alexander, Gun'ko Yurii
Center of Information Optical Technology, ITMO University, 197101 Saint Petersburg, Russia.
Chemistry School, Trinity College Dublin, 2 Dublin, Ireland.
Nanomaterials (Basel). 2021 Jan 5;11(1):109. doi: 10.3390/nano11010109.
Here we report on the development and investigation of a novel multiplex assay model based on polymer microspheres (PMS) encoded with ternary AIS/ZnS quantum dots (QDs). The system was prepared via layer-by-layer deposition technique. Our studies of Förster resonance energy transfer (FRET) between the QD-encoded microspheres and two different cyanine dyes have demonstrated that the QD photoluminescence (PL) quenching steadily increases with a decrease in the QD-dye distance. We have found that the sensitized dye PL intensity demonstrates a clear maximum at two double layers of polyelectrolytes between QDs and Dye molecules on the polymer microspheres. Time resolved PL measurements have shown that the PL lifetime decreases for the QDs and increases for the dyes due to FRET. The designed system makes it possible to record spectrally different bands of FRET-induced dye luminescence with different decay times and thereby allows for the multiplexing by wavelength and photoluminescence lifetimes of the dyes. We believe that PMS encoded with AIS/ZnS QDs have great potential for the development of new highly selective and sensitive sensor systems for multiplex analysis to detect cell lysates and body fluids' representative biomarkers.
在此，我们报告了一种基于用三元AIS/ZnS量子点（QD）编码的聚合物微球（PMS）的新型多重分析模型的开发与研究。该系统通过逐层沉积技术制备。我们对QD编码微球与两种不同菁染料之间的Förster共振能量转移（FRET）的研究表明，QD光致发光（PL）猝灭随着QD - 染料距离的减小而稳定增加。我们发现，在聚合物微球上QD和染料分子之间的两层聚电解质处，敏化染料PL强度呈现出明显的最大值。时间分辨PL测量表明，由于FRET，QD的PL寿命缩短，而染料的PL寿命延长。所设计的系统能够记录具有不同衰减时间的FRET诱导染料发光的光谱不同波段，从而允许通过染料的波长和光致发光寿命进行多重分析。我们认为，用AIS/ZnS QD编码的PMS在开发用于多重分析以检测细胞裂解物和体液代表性生物标志物的新型高选择性和灵敏传感器系统方面具有巨大潜力。
