Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China.
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China.
Int J Mol Sci. 2021 Jan 14;22(2):785. doi: 10.3390/ijms22020785.
Glycerol-3-phosphate acyltransferases (GPATs) play an important role in glycerolipid biosynthesis, and are mainly involved in oil production, flower development, and stress response. However, their roles in regulating plant height remain unreported. Here, we report that Arabidopsis GPAT1 is involved in the regulation of plant height. GUS assay and qRT-PCR analysis in Arabidopsis showed that is highly expressed in flowers, siliques, and seeds. A loss of function mutation in was shown to decrease seed yield but increase plant height through enhanced cell length. Transcriptomic and qRT-PCR data revealed that the expression levels of genes related to gibberellin (GA) biosynthesis and signaling, as well as those of cell wall organization and biogenesis, were significantly upregulated. These led to cell length elongation, and thus, an increase in plant height. Together, our data suggest that knockout of impairs glycerolipid metabolism in Arabidopsis, leading to reduced seed yield, but promotes the biosynthesis of GA, which ultimately enhances plant height. This study provides new evidence on the interplay between lipid and hormone metabolism in the regulation of plant height.
甘油-3-磷酸酰基转移酶（GPATs）在甘油脂质生物合成中发挥重要作用，主要参与油脂生产、花发育和应激反应。然而，它们在调节植物高度方面的作用尚未见报道。在这里，我们报道拟南芥 GPAT1 参与了植物高度的调节。在拟南芥中的 GUS 分析和 qRT-PCR 分析表明， 在花、角果和种子中高度表达。 的功能丧失突变导致种子产量降低，但通过增强细胞长度增加植物高度。转录组和 qRT-PCR 数据分析显示，与赤霉素（GA）生物合成和信号转导相关的基因以及细胞壁组织和生物发生相关的基因的表达水平显著上调。这导致细胞长度伸长，从而增加了植物的高度。总之，我们的数据表明， 敲除会损害拟南芥中的甘油脂质代谢，导致种子产量降低，但促进了 GA 的生物合成，最终增强了植物的高度。这项研究为脂质和激素代谢在调节植物高度方面的相互作用提供了新的证据。
