Wang Ping
Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.
J Fungi (Basel). 2021 Jan 15;7(1):56. doi: 10.3390/jof7010056.
Genetic transformation plays an imperative role in our understanding of the biology in unicellular yeasts and filamentous fungi, such as , and . It also helps to understand the virulence and drug resistance mechanisms of the pathogenic fungus that causes cryptococcosis in health and immunocompromised individuals. Since the first attempt at DNA transformation in this fungus by Edman in 1992, various methods and techniques have been developed to introduce DNA into this organism and improve the efficiency of homology-mediated gene disruption. There have been many excellent summaries or reviews covering the subject. Here we highlight some of the significant achievements and additional refinements in the genetic transformation of species.
基因转化在我们对单细胞酵母和丝状真菌（如酿酒酵母、粗糙脉孢菌和构巢曲霉）生物学的理解中起着至关重要的作用。它也有助于理解引起隐球菌病的致病真菌新生隐球菌在健康个体和免疫功能低下个体中的毒力和耐药机制。自1992年埃德曼首次尝试对该真菌进行DNA转化以来，已经开发了各种方法和技术将DNA导入该生物体，并提高同源介导的基因破坏效率。关于这个主题已经有许多出色的总结或综述。在这里，我们重点介绍新生隐球菌物种基因转化方面的一些重大成就和进一步改进。
