Muñoz-Amatriaín María, Hernandez Javier, Herb Dustin, Baenziger P Stephen, Bochard Anne Marie, Capettini Flavio, Casas Ana, Cuesta-Marcos Alfonso, Einfeldt Claus, Fisk Scott, Genty Amelie, Helgerson Laura, Herz Markus, Hu Gongshe, Igartua Ernesto, Karsai Ildiko, Nakamura Toshiki, Sato Kazuhiro, Smith Kevin, Stockinger Eric, Thomas William, Hayes Patrick
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, United States.
Department of Crop and Soil Science, Oregon State University, Corvallis, OR, United States.
Front Plant Sci. 2020 Nov 9;11:585927. doi: 10.3389/fpls.2020.585927. eCollection 2020.
One option to achieving greater resiliency for barley production in the face of climate change is to explore the potential of winter and facultative growth habits: for both types, low temperature tolerance (LTT) and vernalization sensitivity are key traits. Sensitivity to short-day photoperiod is a desirable attribute for facultative types. In order to broaden our understanding of the genetics of these phenotypes, we mapped quantitative trait loci (QTLs) and identified candidate genes using a genome-wide association studies (GWAS) panel composed of 882 barley accessions that was genotyped with the Illumina 9K single-nucleotide polymorphism (SNP) chip. Fifteen loci including 5 known and 10 novel QTL/genes were identified for LTT-assessed as winter survival in 10 field tests and mapped using a GWAS meta-analysis. , , and were major drivers of LTT, and candidate genes were identified for . The principal determinants of vernalization sensitivity were , , and . deletions conferred insensitive or intermediate sensitivity to vernalization. A subset of accessions with maximum LTT were identified as a resource for allele mining and further characterization. Facultative types comprised a small portion of the GWAS panel but may be useful for developing germplasm with this growth habit.
面对气候变化，提高大麦生产恢复力的一种选择是探索冬性和兼性生长习性的潜力：对于这两种类型，耐低温性（LTT）和春化敏感性是关键性状。对短日光周期的敏感性是兼性类型的一个理想特性。为了拓宽我们对这些表型遗传学的理解，我们利用一个由882份大麦种质组成的全基因组关联研究（GWAS）群体，通过Illumina 9K单核苷酸多态性（SNP）芯片进行基因分型，绘制了数量性状位点（QTL）并鉴定了候选基因。通过在10次田间试验中评估为冬季存活率的LTT，利用GWAS荟萃分析鉴定了15个位点，包括5个已知的和10个新的QTL/基因，并进行了定位。 、 和 是LTT的主要驱动因素，并鉴定了 的候选基因。春化敏感性的主要决定因素是 、 和 。 缺失赋予了对春化不敏感或中等敏感性。鉴定出具有最大LTT的一部分种质作为等位基因挖掘和进一步表征的资源。兼性类型在GWAS群体中占一小部分，但可能有助于培育具有这种生长习性的种质。
