膳食绿原酸通过激活Nrf2改善糖尿病小鼠的氧化应激并增强内皮功能。

Dietary chlorogenic acid ameliorates oxidative stress and improves endothelial function in diabetic mice via Nrf2 activation.

作者信息

Wang Dan, Hou Jixin, Wan Jindong, Yang Yi, Liu Sen, Li Xiaoqing, Li Wenzhang, Dai Xiaozhen, Zhou Peng, Liu Weihua, Wang Peijian

机构信息

Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, Chengdu, Sichuan, China.

Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, Sichuan, China.

出版信息

J Int Med Res. 2021 Jan;49(1):300060520985363. doi: 10.1177/0300060520985363.

OBJECTIVES

Chlorogenic acid (CGA) is an antioxidant dietary factor. We investigated the effects of CGA on endothelial cell dysfunction in diabetic mice and the mechanistic role of nuclear factor erythroid-related factor 2 (Nrf2) in the antioxidant effect of CGA.

METHODS

Diabetic (db/db) mice were fed normal chow or chow containing 0.02% CGA for 12 weeks. Human umbilical vein endothelial cells (HUVECs) and mouse aortas were treated with normal or high glucose.

RESULTS

CGA treatment induced upregulation of Nrf2 in HUVECs in a dose-dependent manner. CGA pretreatment prevented reactive oxygen species generation and preserved nitric oxide bioavailability in HUVECs and aortas from wild-type but not Nrf2 mice. CGA improved endothelium-dependent relaxation in high glucose-treated aortas from wild-type and db/db mice, but not Nrf2 mice. Dietary CGA improved endothelium-dependent relaxation in db/db mice.

CONCLUSIONS

CGA ameliorates endothelial dysfunction in diabetic mice through activation of the Nrf2 anti-oxidative pathway.

目的

绿原酸（CGA）是一种具有抗氧化作用的膳食因子。我们研究了CGA对糖尿病小鼠内皮细胞功能障碍的影响以及核因子红系相关因子2（Nrf2）在CGA抗氧化作用中的机制性作用。

方法

将糖尿病（db/db）小鼠分为两组，分别喂食普通饲料或含0.02% CGA的饲料，持续12周。用人脐静脉内皮细胞（HUVECs）和小鼠主动脉进行正常或高糖处理。

结果

CGA处理以剂量依赖的方式诱导HUVECs中Nrf2的上调。CGA预处理可防止野生型而非Nrf2小鼠的HUVECs和主动脉中活性氧的产生，并保留一氧化氮的生物利用度。CGA改善了野生型和db/db小鼠高糖处理主动脉中的内皮依赖性舒张，但对Nrf2小鼠无效。膳食CGA改善了db/db小鼠的内皮依赖性舒张。