Tabasum Wajeeda, Sreenivas Ara, Bheemavarapu Kesav Kumar, Golla Tirupathi Rao, Gaur Ajay
Conservation Genetics Lab, LaCONES, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
Mitochondrial DNA B Resour. 2016 Sep 5;1(1):621-622. doi: 10.1080/23802359.2016.1214543.
The complete mitochondrial genome of sequence 16,859 bp of Indian clouded leopard () has been sequenced using next generation sequencing technology Torrent platform. The complete mitochondrial genome sequence of clouded leopard consists of 13 protein-coding, 22 tRNA, and two rRNA genes and a control region (CR). The mitochondrial genome is relatively similar to other felid mitochondrial genomes with respect to gene arrangement, composition, tRNA structures and skews of AT/GC bases to be typical of those reported for other mammals. The nucleotide composition of the genome shows that there is more A-T% than G-C% on the positive strand as revealed by positive AT and CG skews. The base composition of the mitochondrial genome of clouded leopard is as follows: A, 5362 bp (31.8%); C, 4560bp (27.0%); G, 2475 bp (14.6%); T, 4462 bp (26.4%). Most of the genes have ATG initiation codons, except ND1, ND2, ND3, ND4, ND6, and CYTB (ATA start codon).
已使用新一代测序技术Torrent平台对印度云豹（）16,859bp的完整线粒体基因组进行了测序。云豹的完整线粒体基因组序列由13个蛋白质编码基因、22个tRNA基因、两个rRNA基因和一个控制区（CR）组成。线粒体基因组在基因排列、组成、tRNA结构以及AT/GC碱基偏斜方面与其他猫科动物的线粒体基因组相对相似，是其他哺乳动物所报道的典型特征。基因组的核苷酸组成表明，如正向的AT和CG偏斜所示，正链上的A-T%多于G-C%。云豹线粒体基因组的碱基组成如下：A，5362bp（31.8%）；C，4560bp（27.0%）；G，2475bp（14.6%）；T，4462bp（26.4%）。除ND1、ND2、ND3、ND4、ND6和CYTB（ATA起始密码子）外，大多数基因具有ATG起始密码子。
