Tabasum Wajeeda, Ara Sreenivas, Rai Niraj, Thangaraj Kumarasamy, Gaur Ajay
Conservation Genetics Lab, LaCONES, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
Evolutionary Biology and Medical Genetics Lab, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
Mitochondrial DNA B Resour. 2016 Sep 5;1(1):619-620. doi: 10.1080/23802359.2016.1214541.
The complete mitochondrial genome sequence 17,059 bp of Asiatic lion ( has been sequenced with the use of next generation sequencing technology using Ion Torrent platform. The complete mitochondrial genome sequence of Asiatic lion consists of 13 protein-coding, 22 tRNA, and two rRNA genes, and 1 control region (CR). The mitochondrial genome is relatively similar to other felid mitochondrial genomes with respect to gene arrangement, composition, tRNA structures and skews of AT/GC bases to be typical of those reported for other mammals. The nucleotide composition of Asiatic lion mitogenome shows that there is more A-T% than G-C% on the positive strand as revealed by positive AT and CG skews. The overall base composition is 31.9% of A, 27.2% of C, 14.5% of G, and 26.2% of T. Most of the genes have ATA start codons, except ND1, COX2, ATP8, ATP6, ND4, and ND5 have ATG start codons.
利用离子激流平台的下一代测序技术，对亚洲狮17,059 bp的完整线粒体基因组序列进行了测序。亚洲狮的完整线粒体基因组序列由13个蛋白质编码基因、22个tRNA基因、两个rRNA基因和1个控制区（CR）组成。线粒体基因组在基因排列、组成、tRNA结构以及AT/GC碱基偏斜方面与其他猫科动物的线粒体基因组相对相似，是其他哺乳动物所报道的典型特征。亚洲狮有丝分裂基因组的核苷酸组成表明，正链上A-T%比G-C%多，这由正的AT和CG偏斜所揭示。总体碱基组成为：A占31.9%，C占27.2%，G占14.5%，T占26.2%。大多数基因有ATA起始密码子，除了ND1、COX2、ATP8、ATP6、ND4和ND5有ATG起始密码子。
