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通过低深度双端Illumina测序快速恢复印度主要鲤鱼的完整线粒体基因组

Rapid recovery of complete mitogenome of Indian major carp, from low depth paired end Illumina sequencing.

作者信息

Sahoo L, Bit A, Meher P K, Murmu K, Sundaray J K, Das P

机构信息

Fish Genetics and Biotechnology Division, ICAR-CIFA, Kausalyaganga, Bhubaneswar, India.

出版信息

Mitochondrial DNA B Resour. 2017 Mar 10;2(1):155-156. doi: 10.1080/23802359.2017.1298413.

Abstract

Here we report the reconstruction of the catla () complete mitochondrial genome sequence from low depth paired end Illumina sequencing. The genome is of 16,597 bp in size. Similar to other vertebrate mtgenomes, it consists of 13 protein-coding genes, 22 tRNAs, 2 rRNAs and a putative control region. The present mtgenome is 3 bp longer than the earlier reported catla mtgenome from our laboratory. Majority of the mitochondrial genes are encoded by the H-strand. Phylogenetics analysis revealed that is closer to than other labeo species. Present study demonstrated the power of next generation sequencing towards hassle free and rapid sequencing of mitochondrial genomes of non-model organisms.

摘要

在此,我们报告了通过低深度双末端Illumina测序对卡特拉鱼()完整线粒体基因组序列的重建。该基因组大小为16,597 bp。与其他脊椎动物线粒体基因组相似,它由13个蛋白质编码基因、22个tRNA、2个rRNA和一个假定的控制区域组成。目前的线粒体基因组比我们实验室之前报道的卡特拉鱼线粒体基因组长3 bp。大多数线粒体基因由重链编码。系统发育分析表明,与其他野鲮属物种相比, 与 更接近。本研究证明了下一代测序技术在非模式生物线粒体基因组无麻烦快速测序方面的强大能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/614d/7799541/7616a5504dfd/TMDN_A_1298413_F0001_B.jpg

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