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利用Illumina双末端测序对龙眼(Lour.)完整叶绿体基因组进行表征

Characterization of the complete chloroplast genome of longan ( Lour.) using illumina paired-end sequencing.

作者信息

Wang Kangyu, Li Li, Zhao Mingzhu, Li Shaokun, Sun Honghua, Lv Yanxi, Wang Yi

机构信息

College of Life Science, Jilin Agricultural University, Changchun, China.

出版信息

Mitochondrial DNA B Resour. 2017 Dec 7;2(2):904-906. doi: 10.1080/23802359.2017.1413310.

Abstract

Longan ( Lour.), as a kind of commercial fruit tree in the family Sapindaceae, is widely cultivated in South Asia. In this study, we obtained the complete chloroplast genome sequence of longan using Illumina paired-end sequencing. It has 160,833 bp in length, containing a pair of IR regions (28,428 bp) separated by a small single-copy region (18,270 bp) and a large single-copy region (85,707 bp). The overall GC contents of the chloroplast genome were 37.8%. This circular genome contains 130 annotated genes, including 85 protein-coding genes, 37 tRNAs and 8 rRNAs. The phylogenetic analysis using maximum-likelihood (ML) and neighbour-joining (NJ) method showed that longan has the closest relationship with , and This complete chloroplast genomes can be subsequently used for the genetic breeding of this valuable species.

摘要

龙眼(Lour.)是无患子科的一种商业果树,在南亚广泛种植。在本研究中,我们利用Illumina双末端测序获得了龙眼的完整叶绿体基因组序列。其长度为160,833 bp,包含一对由小单拷贝区域(18,270 bp)和大单拷贝区域(85,707 bp)分隔的反向重复区域(28,428 bp)。叶绿体基因组的总体GC含量为37.8%。这个环状基因组包含130个注释基因,包括85个蛋白质编码基因、37个tRNA和8个rRNA。使用最大似然法(ML)和邻接法(NJ)进行的系统发育分析表明,龙眼与 、 和 关系最为密切。这个完整的叶绿体基因组随后可用于该珍贵物种的遗传育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be82/7799724/7a1bccf009d8/TMDN_A_1413310_F0001_C.jpg

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