Ginting Chrismis Novalinda, Lister I Nyoman Ehrich, Girsang Ermi, Widowati Wahyu, Yusepany Dewani Tediana, Azizah Alya Mardhotillah, Kusuma Hanna Sari Widya
Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1, Medan 20118, North Sumatera, Indonesia.
Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri No. 65, Bandung 40163, West Java, Indonesia.
Heliyon. 2021 Jan 4;7(1):e05620. doi: 10.1016/j.heliyon.2020.e05620. eCollection 2021 Jan.
Acetaminophen (APAP) is a widely used analgesic, but it may cause liver injury ( via oxidative stress that induced by -acetyl--benzoquinone imine (NAPQI) in long term usage or overdose. Multiple inflammatory mediators were also found to contribute for this effect. Many medicinal plants was known for its antioxidant and anti-inflammatory activities and one of them is Red betel ( Ruiz and Pav) from Indonesia. In this study, the red betel leaves extract (RBLE) protective effect against APAP-induced HepG2 cells was determined. APAP-induced HepG2 as hepatotoxicity cell model was treated with RBLE at 25 and 100 μg/mL. Protective effects of RBLE toward hepatotoxicity were evaluated by several parameters: tumor necrosis factor-α (TNF-α) concentration, reactive oxygen species (ROS) level, live cells percentage, apoptotic cells percentage, necrotic cells percentage, death cells percentage, CYP2E1 and GPX gene expression. The RBLE treatments (both 25 and 100 μg/mL) increased CYP2E1 and GPX gene expression also live cells percentage, while decreased ROS level, TNF-α concentration, also the percentage of death and necrotic cells. Red Betel leaves ethanol extract has hepatoprotective effect via anti-inflammatory, anti-necrotic, and antioxidant potency in liver injury model.
对乙酰氨基酚（APAP）是一种广泛使用的镇痛药，但长期使用或过量服用时，它可能会通过由N - 乙酰 - 对苯醌亚胺（NAPQI）诱导的氧化应激导致肝损伤。还发现多种炎症介质也促成了这种效应。许多药用植物因其抗氧化和抗炎活性而闻名，其中之一是来自印度尼西亚的红槟榔（Ruiz和Pav）。在本研究中，测定了红槟榔叶提取物（RBLE）对APAP诱导的HepG2细胞的保护作用。以25和100μg/mL的RBLE处理作为肝毒性细胞模型的APAP诱导的HepG2细胞。通过几个参数评估RBLE对肝毒性的保护作用：肿瘤坏死因子-α（TNF-α）浓度、活性氧（ROS）水平、活细胞百分比、凋亡细胞百分比、坏死细胞百分比、死亡细胞百分比、CYP2E1和GPX基因表达。RBLE处理（25和100μg/mL）均增加了CYP2E1和GPX基因表达以及活细胞百分比，同时降低了ROS水平、TNF-α浓度以及死亡和坏死细胞的百分比。在肝损伤模型中，红槟榔叶乙醇提取物通过抗炎、抗坏死和抗氧化作用具有肝保护作用。
