State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
Chem Commun (Camb). 2021 Feb 18;57(14):1798-1801. doi: 10.1039/d0cc07954e. Epub 2021 Jan 21.
Lysine ε-N-benzoylation is a recently identified PTM occurring on histone proteins, and herein we genetically encoded ε-N-benzoyl-lysine (BzK) into recombinant proteins in E. coli and mammalian cells, and applied it for the modification of histone proteins and the analysis of sirtuin debenzoylase activity.
赖氨酸 ε-N-苯甲酰化是最近在组蛋白上发现的一种翻译后修饰（PTM），在此我们在大肠杆菌和哺乳动物细胞中通过基因编码将 ε-N-苯甲酰基-赖氨酸（BzK）导入重组蛋白，并将其应用于组蛋白的修饰和去苯甲酰基酶活性的分析。
