Wang Si-Han, Lee Shiao-Pieng, Yang Chung-Wei, Lo Chun-Min
Department of Biomedical Engineering, National Yang-Ming University, Taipei 11221, Taiwan.
Division of Oral and Maxillofacial Surgery, Department of Dentistry, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan.
Materials (Basel). 2021 Jan 18;14(2):441. doi: 10.3390/ma14020441.
Magnesium alloys with coatings have the potential to be used for bone substitute alternatives since their mechanical properties are close to those of human bone. However, the surface modification of magnesium alloys to increase the surface biocompatibility and reduce the degradation rate remains a challenge. Here, FHA-Mg scaffolds were made of magnesium alloys and coated with fluorohydroxyapatite (FHA). Human mesenchymal stem cells (hMSCs) were cultured on FHA-Mg scaffolds and cell viability, proliferation, and osteogenic differentiation were investigated. The results showed that FHA-Mg scaffolds display a nano-scaled needle-like structure of aggregated crystallites on their surface. The average Mg concentration in the conditioned media collected from FHA-Mg scaffolds (5.8-7.6 mM) is much lower than those collected from uncoated, Mg(OH)-coated, and hydroxyapatite (HA)-coated samples (32.1, 17.7, and 21.1 mM, respectively). In addition, compared with hMSCs cultured on a culture dish, cells cultured on FHA-Mg scaffolds demonstrated better proliferation and comparable osteogenic differentiation. To eliminate the effect of osteogenic induction medium, hMSCs were cultured on FHA-Mg scaffolds in culture medium and an approximate 66% increase in osteogenic differentiation was observed three weeks later, indicating a significant effect of the nanostructured surface of FHA-Mg scaffolds on hMSC behaviors. With controllable Mg release and favorable mechanical properties, porous FHA-Mg scaffolds have a great potential in cell-based bone regeneration.
具有涂层的镁合金因其机械性能与人体骨骼相近而有潜力用作骨替代物。然而，对镁合金进行表面改性以提高其表面生物相容性并降低降解速率仍是一项挑战。在此，FHA-Mg支架由镁合金制成，并涂覆有氟羟基磷灰石（FHA）。将人间充质干细胞（hMSCs）培养在FHA-Mg支架上，并研究细胞活力、增殖和成骨分化情况。结果表明，FHA-Mg支架表面呈现出纳米级的针状聚集微晶结构。从FHA-Mg支架收集的条件培养基中的平均镁浓度（5.8 - 7.6 mM）远低于从未涂层、涂覆Mg(OH)和涂覆羟基磷灰石（HA）的样品中收集的浓度（分别为32.1、17.7和21.1 mM）。此外，与在培养皿上培养的hMSCs相比，在FHA-Mg支架上培养的细胞表现出更好的增殖能力和成骨分化能力。为消除成骨诱导培养基的影响，将hMSCs在培养基中培养于FHA-Mg支架上，三周后观察到成骨分化增加了约66%，这表明FHA-Mg支架的纳米结构表面对hMSC行为有显著影响。由于具有可控的镁释放和良好的机械性能，多孔FHA-Mg支架在基于细胞的骨再生方面具有巨大潜力。
