Hofmann H, Tuma W, Heinz F X, Kunz C
Institut für Virologie, Universität Wien.
Wien Klin Wochenschr. 1988 Jan 22;100(2):52-5.
Detection of hepatitis B viral DNA (HBV-DNA) is the most reliable test for infectivity of a patient's serum. HBV-DNA was detected by spot hybridization on nylon membrane. HBV-DNA was detected during the first or second week of jaundice in 22/133 (16.5%) sera from patients with acute hepatitis who did not develop chronic disease later on. However, in patients with acute hepatitis who later on developed the chronic form, HBV-DNA was found in 12/12 (100%) cases in the first or second week after onset. In other patients who had chronic hepatitis for longer than one year HBV-DNA was detected in 92/113 (81.4%) sera when HBe antigen was also detectable and in 11/104 (10.6%) when HBe antibodies were found. HBV-DNA was present in 32/38 (84.2%) sera from healthy antigen carriers when HBe antigen was detected and only in 2/173 (1.2%) sera of HBe antibody positive HBs antigen carriers. The highest levels of HBV-DNA were found in patients with chronic hepatitis B infection who were found in patients with chronic hepatitis B infection who were on haemodialysis therapy. Almost all had HBe antigen and HBV-DNA was also detectable in 82/84 (97%) sera. 11/28 (39.3%) sera from patients with chronic hepatitis B as well as HIV infection showed HBV-DNA. Detection of HBV-DNA has significantly improved the accuracy of diagnosis of hepatitis B viral infection.
检测乙型肝炎病毒DNA（HBV-DNA）是判断患者血清传染性最可靠的检测方法。通过尼龙膜斑点杂交法检测HBV-DNA。在急性肝炎患者且后期未发展为慢性病的133份血清中，有22份（16.5%）在黄疸出现的第一周或第二周检测到HBV-DNA。然而，在后期发展为慢性肝炎的急性肝炎患者中，发病后第一周或第二周有12/12例（100%）检测到HBV-DNA。在其他慢性肝炎病程超过一年的患者中，当可检测到HBe抗原时，113份血清中有92份（81.4%）检测到HBV-DNA；当发现HBe抗体时，104份血清中有11份（10.6%）检测到HBV-DNA。在检测到HBe抗原的健康抗原携带者的38份血清中，有32份（84.2%）存在HBV-DNA，而在HBe抗体阳性的HBs抗原携带者的173份血清中，只有2份（1.2%）存在HBV-DNA。在接受血液透析治疗的慢性乙型肝炎感染患者中发现HBV-DNA水平最高。几乎所有患者都有HBe抗原，84份血清中有82份（97%）也可检测到HBV-DNA。28份慢性乙型肝炎合并HIV感染患者的血清中有11份（39.3%）显示有HBV-DNA。HBV-DNA的检测显著提高了乙型肝炎病毒感染诊断的准确性。
