School of Materials Science and Engineering, University of Jinan, Jinan 250022, PR China.
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
J Hazard Mater. 2021 Jun 5;411:125106. doi: 10.1016/j.jhazmat.2021.125106. Epub 2021 Jan 13.
A fluorescent assay for total antioxidant capacity (TAC) detection based on the light-responsive oxidase-like activity of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) has been developed. Thiamine (TH) as the peroxidase substrate usually works at alkaline conditions and thus limits its practical applications. Here, by utilization the light-responsive oxidase-like activity of BSA-AuNCs, TH is oxidized to fluorescent thiochrome under neutral condition in two minutes due to the single oxygen generated by BSA-AuNCs upon light irradiation. After the introduction of antioxidants into the BSA-AuNCs-TH system, the formation of thiochrome is inhibited resulting in the fluorescence decrease. On the basis of the above facts, BSA-AuNCs-TH-based assay has been fabricated and applied successfully to detect antioxidants and the TAC of vitamin C tablets as well as some commercial fruit juice with satisfied results. This work may provide novel insights into developing light-responsive nanozymes-based fluorescent assays.
基于牛血清白蛋白稳定的金纳米簇（BSA-AuNCs）的光响应氧化酶样活性，开发了一种用于总抗氧化能力（TAC）检测的荧光分析方法。作为过氧化物酶底物的硫胺素（TH）通常在碱性条件下工作，因此限制了其实际应用。在这里，利用 BSA-AuNCs 的光响应氧化酶样活性，由于 BSA-AuNCs 在光照下产生的单氧，TH 在中性条件下两分钟内被氧化为荧光噻嗪。在向 BSA-AuNCs-TH 体系中引入抗氧化剂后，噻嗪的形成受到抑制，导致荧光强度降低。基于上述事实，构建了基于 BSA-AuNCs-TH 的测定方法，并成功地用于检测抗氧化剂和维生素 C 片剂以及一些商业果汁的 TAC，结果令人满意。这项工作可能为开发基于光响应纳米酶的荧光分析方法提供新的思路。
