Liu LiangLiang, Grillo Fabiana, Canfarotta Francesco, Whitcombe Michael, Morgan Stephen P, Piletsky Sergey, Correia Ricardo, He ChenYang, Norris Andrew, Korposh Serhiy
Optics and Photonics Group, University of Nottingham, Nottingham, UK.
Department of Chemistry, University of Leicester, Leicester, UK.
Biosens Bioelectron. 2021 Apr 1;177:113002. doi: 10.1016/j.bios.2021.113002. Epub 2021 Jan 16.
Butyrylfentanyl is a new designer drug reported with growing use and related deaths. Routine toxicological analyses of this novel synthetic opioid drug have not been established yet. This work reports a fibre optic sensor that measures carboxyl-fentanyl which is the major metabolite of butyrylfentanyl presented in blood, providing a promising tool for detecting butyrylfentanyl intoxication. A long period fibre grating (LPG) sensor array operating at phase-matching condition is deployed in combination with a state-of-the-art molecular imprinting technique. Nano-sized molecularly imprinted polymers (nanoMIPs) are synthesised via a solid-phase approach and coated on the surface of an LPG array. An LPG array consists of two parts: a detection and a reference LPG. The former is functionalised with nanoMIPs prior to the measurements, whilst the latter is used to take into account the temperature response of the detection LPG. The developed sensor exhibits a gradual response over increasing concentrations of carboxyl-fentanyl from 0 to 1000 ng/mL with a minimal detected concentration of 50 ng/mL, that corresponds to a wavelength shift of 1.20 ± 0.2 nm. The Langmuir adsorption isotherm is applied to fit the analytical data which reveal a binding constant of 2.03 μM. The developed sensor shows high selectivity in detecting carboxyl-fentanyl among other drugs and potential interferents including morphine, cocaine, glucose and albumin. It shows a certain degree of cross-response to fentanyl which shares the same binding sites as carboxyl-fentanyl and therefore can be potentially used to detect fentanyl.
丁酰芬太尼是一种新型合成毒品，其使用和相关死亡案例不断增加。目前尚未建立对这种新型合成阿片类药物的常规毒理学分析方法。本文报道了一种光纤传感器，可测量血液中丁酰芬太尼的主要代谢产物羧基芬太尼，为检测丁酰芬太尼中毒提供了一种有前景的工具。一种在相位匹配条件下工作的长周期光纤光栅（LPG）传感器阵列与先进的分子印迹技术相结合使用。通过固相方法合成纳米尺寸的分子印迹聚合物（nanoMIPs），并将其涂覆在LPG阵列表面。LPG阵列由两部分组成：一个检测LPG和一个参考LPG。在测量之前，前者用nanoMIPs进行功能化，而后者用于考虑检测LPG的温度响应。所开发的传感器在羧基芬太尼浓度从0到1000 ng/mL增加时呈现出逐渐响应，最低检测浓度为50 ng/mL，对应波长偏移为1.20±0.2 nm。应用朗缪尔吸附等温线拟合分析数据，揭示结合常数为2.03 μM。所开发的传感器在检测羧基芬太尼时对其他药物和潜在干扰物（包括吗啡、可卡因、葡萄糖和白蛋白）具有高选择性。它对芬太尼有一定程度的交叉响应，芬太尼与羧基芬太尼共享相同的结合位点，因此有可能用于检测芬太尼。
