Soeyono Gunanti, Dahlan Kiagus, Purba Melpa Susanti, Widhyari Sus Dherthi, Soesatyoratih Rr, Teng Thang Shi, Budiarti Lieonny, Wai Ho Kin, Kosat Agatha
Department of Veterinary Clinic Reproduction and Pathology, Division of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Veterinary Paramedic Study Program, Vocational School, Bogor Agriculture University, Bogor, Indonesia.
Vet World. 2020 Dec;13(12):2635-2642. doi: 10.14202/vetworld.2020.2635-2642. Epub 2020 Dec 11.
Calcium phosphate bioceramics have been used for at least a decade, and many investigations have focused on the use of hydroxyapatite (HA) derivative in the regeneration of bone defects. Biphasic calcium phosphate (BCP) is a biomaterial composed of HA and beta-tricalcium phosphate (BCP), with a structure similar to bone. The aim of the study was to determine the influence of the BCP/alginate scaffold on tissue growth, blood, the lungs, and the electrical activity of the heart during bone healing in the tibia of pig.
Three pigs were implanted with BCP/alginate scaffolds in the tibias. Pigs were acclimatized and treated with antibiotics and anthelminthic drugs 14 days before implantation. Each pig was implanted with a BCP/alginate scaffold in the right tibia and a defect without the implant was made in the left tibia as the control. Radiographic images of the tibia were captured 0, 7, 30, and 60 days after the operation. Erythrograms, radiography of the lungs, and electrocardiogram (ECG) recordings were done 0, 30, and 60 days after the operation.
Radiographic evaluations showed that the implant and peri-implant density of BCP decreased throughout the process of bone healing. The erythrogram profile indicated that a substantial amount of time (60 days) was required to adapt and return to pre-operative conditions. No significant differences in ECG recordings or pulmonary radiography were detected.
The BCP/alginate scaffold did not induce a faster recovery rate from the bone defect compared to the control with no implant. However, the BCP/alginate scaffold was biodegradable, bioresorbable, and non-toxic.
磷酸钙生物陶瓷已被使用至少十年，许多研究聚焦于羟基磷灰石（HA）衍生物在骨缺损再生中的应用。双相磷酸钙（BCP）是一种由HA和β-磷酸三钙组成的生物材料，其结构与骨相似。本研究的目的是确定BCP/藻酸盐支架对猪胫骨骨愈合过程中组织生长、血液、肺部及心脏电活动的影响。
三只猪的胫骨植入BCP/藻酸盐支架。在植入前14天，猪进行适应性饲养，并给予抗生素和驱虫药治疗。每只猪的右胫骨植入BCP/藻酸盐支架，左胫骨制造无植入物的缺损作为对照。术后0、7、30和60天拍摄胫骨的X线图像。术后0、30和60天进行血常规、肺部X线检查和心电图（ECG）记录。
X线评估显示，在骨愈合过程中，BCP植入物及其周围的密度逐渐降低。血常规结果表明，需要相当长的时间（60天）才能适应并恢复到术前状态。未检测到心电图记录或肺部X线检查有显著差异。
与无植入物的对照相比，BCP/藻酸盐支架并未使骨缺损的恢复速度更快。然而，BCP/藻酸盐支架具有可生物降解、可生物吸收且无毒的特性。
