Khochamit Nalisa, Siripornadulsil Surasak, Sukon Peerapol, Siripornadulsil Wilailak
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand.
Vet World. 2020 Dec;13(12):2663-2672. doi: 10.14202/vetworld.2020.2663-2672. Epub 2020 Dec 16.
The aim of the study was to determine the potentials and effects of and lactic acid bacteria (LAB) as probiotics on broiler growth, health, and infection.
To evaluate the inoculum size applicable for broilers, 1-day-old broilers were orally fed fresh cultures of single strains and a KKU213/ NP6 mixture at 10 and 10 colony-forming unit (CFUs)/mL/chick. The body weight gain (BWG), contamination level and total and LAB abundances in the crop and intestine were measured. Subsequently, 1-day-old broilers were orally fed of KKU213, CH403, and SH8 at 10 CFUs/mL, followed by inulin. After 35 days, the BWG, and LAB abundances in the cecum, blood parameters, and KKU213 colonization were assessed.
The broilers fed single strains or KKU213+NP6 exhibited a higher BWG and a higher crop LAB abundance than the controls (p<0.05). Probiotic feeding decreased the intestinal abundance and correspondingly increased the LAB abundance. The broilers fed the mixed culture (KKU213+CH403+SH8) followed by prebiotics showed lower mortality, higher blood high-density lipoprotein levels, and lower blood uric acid levels than the controls (p<0.0004). Probiotic feeding significantly increased the and LAB counts (p<0.05). A CE330 isolate obtained from the cecum after 35 days of KKU213 feeding was closely related to KKU213.
KKU213 is a potent probiotic strain that can survive, colonize and reduce infection in broilers and improve their growth and health. This strain, combined with different LAB can act synergistically in the gut and promote broiler growth.
本研究旨在确定芽孢杆菌属和乳酸菌（LAB）作为益生菌对肉鸡生长、健康及感染的潜力和影响。
为评估适用于肉鸡的接种量，给1日龄肉鸡经口投喂单菌株新鲜培养物以及10⁸和10⁹菌落形成单位（CFU）/毫升/只鸡的KKU213/NP6混合物。测量体重增加（BWG）、沙门氏菌污染水平以及嗉囊和肠道中沙门氏菌和LAB的总丰度。随后，给1日龄肉鸡经口投喂10⁸CFU/毫升的KKU213、CH403和嗜酸乳杆菌SH8，之后投喂菊粉。35天后，评估盲肠中的BWG、沙门氏菌和LAB丰度、血液参数以及KKU213定植情况。
与对照组相比，投喂单菌株或KKU213 + NP6的肉鸡表现出更高的BWG和更高的嗉囊LAB丰度（p<0.05）。益生菌投喂降低了肠道沙门氏菌丰度，并相应增加了LAB丰度。先投喂混合培养物（KKU213 + CH403 + SH8）再投喂益生元的肉鸡与对照组相比，死亡率更低、血液高密度脂蛋白水平更高且血液尿酸水平更低（p<0.0004）。益生菌投喂显著增加了沙门氏菌和LAB计数（p<0.05）。KKU213投喂35天后从盲肠分离出的CE330菌株与芽孢杆菌属KKU213密切相关。
KKU213是一种有效的益生菌菌株，能够在肉鸡体内存活、定植并减少沙门氏菌感染，改善其生长和健康状况。该菌株与不同的LAB组合可在肠道中协同作用，促进肉鸡生长。
