Mehrabani Mehrnaz, Ansari-Asl Zeinab, Rostamzadeh Farzaneh, Jafarinejad-Farsangi Saeideh, Hashemi Mahnaz Sadat, Sheikholeslami Mozhgan, Neisi Zeinab
Cardiovascular Research Centre, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman Iran.
Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz Iran.
Turk J Chem. 2020 Apr 1;44(2):472-485. doi: 10.3906/kim-1910-63. eCollection 2020.
Nowadays, metal-organic frameworks (MOFs) have emerged as promising tools for different biological applications and therefore, efforts are ongoing to develop more biocompatible MOFs-based nanocomposites. We aimed to fabricate some new conductive nanocomposites of polypyrrole and cobalt-MOF with different weight percentages (PPy/x%Co-MOF) using the solution mixing method and characterize them through FT-IR (Fourier-transform infrared), PXRD (powder X-ray diffraction), SEM (scanning electron microscope), and TEM (transmission electron microscope) techniques. The biocompatibility of nanocomposites was assessed by haemolytic, cytotoxic, and quantitative reverse transcription PCR (qRT-PCR) assays. FT-IR and PXRD results revealed that nanocomposites consisted of pure MOFs and PPy. Moreover, SEM results indicated their spherical morphology along with an average diameter of 190 nm. (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a concentration, and percentagedependent cytotoxic effect of the nanocomposites on some cell lines including 3T3 fibroblasts, MCF-7, and J774.A1 macrophages. Haematological toxicity of PPy/x%Co-MOF composites was less than 7% in most concentrations. Furthermore, PPy/x%Co-MOF composites did not show any significant effect on the expression of cyclooxygenase-2( COX-2) and inducible nitric oxide synthase( iNOS) genes. In sum, regarding the haemolytic, proinflammatory, and cytotoxic tests, prepared nanocomposite demonstrated the reasonable in vitro biocompatibility which may be considered as a hopeful platform for further investigations including clinical applications.
如今，金属有机框架材料（MOFs）已成为用于不同生物应用的有前景的工具，因此，人们正在努力开发更多具有生物相容性的基于MOFs的纳米复合材料。我们旨在使用溶液混合法制备一些不同重量百分比的聚吡咯与钴-MOF的新型导电纳米复合材料（PPy/x%Co-MOF），并通过傅里叶变换红外光谱（FT-IR）、粉末X射线衍射（PXRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）技术对其进行表征。通过溶血、细胞毒性和定量逆转录聚合酶链反应（qRT-PCR）分析评估纳米复合材料的生物相容性。FT-IR和PXRD结果表明，纳米复合材料由纯MOFs和PPy组成。此外，SEM结果显示它们具有球形形态，平均直径为190 nm。噻唑蓝（MTT）分析表明，纳米复合材料对包括3T3成纤维细胞、MCF-7和J774.A1巨噬细胞在内的一些细胞系具有浓度和百分比依赖性的细胞毒性作用。在大多数浓度下，PPy/x%Co-MOF复合材料的血液学毒性小于7%。此外，PPy/x%Co-MOF复合材料对环氧合酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）基因的表达没有任何显著影响。总之，关于溶血、促炎和细胞毒性测试，制备的纳米复合材料表现出合理的体外生物相容性，这可被视为进一步研究（包括临床应用）的一个有希望的平台。
