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Can J Vet Res. 1988 Jan;52(1):37-41.
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Antigens of Coxiella burnetii. I. Extraction of antigens with non-aqueous organic solvents.伯氏考克斯氏体抗原。I. 用非水有机溶剂提取抗原。
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2
A capillary-tube test for antibody against Coxiella burnetii in human, guinea pig, and sheep sera.一种用于检测人、豚鼠和绵羊血清中抗伯氏考克斯氏体抗体的毛细管试验。
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[Continuous multiplication of Coxiella burnetii through persisting infection in buffalo-green monkey (BGM) cell cultures].[通过在水牛绿猴（BGM）细胞培养物中的持续感染实现伯氏考克斯氏体的连续增殖]
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ELISA in the Q fever diagnosis.ELISA在Q热诊断中的应用。
Arch Roum Pathol Exp Microbiol. 1983 Oct-Dec;42(4):283-8.
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[Epidemiology of Q fever in Hesse: studies using enzyme immunoassay and the complement fixation reaction].
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Early diagnosis of Q fever: detection of immunoglobulin M by radioimmunoassay and enzyme immunoassay.Q热的早期诊断：通过放射免疫测定和酶免疫测定检测免疫球蛋白M
Eur J Clin Microbiol. 1984 Dec;3(6):550-3. doi: 10.1007/BF02013617.
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A rapid immunofluorescent procedure for serodiagnosis of Q fever in mice, guinea pigs, sheep, and humans.一种用于小鼠、豚鼠、绵羊和人类Q热血清学诊断的快速免疫荧光程序。
Diagn Immunol. 1983;1(1):33-8.
8
Detection and persistence of specific IgM antibody to Coxiella burnetii by enzyme-linked immunosorbent assay: a comparison with immunofluorescence and complement fixation tests.通过酶联免疫吸附测定法检测伯纳特立克次体特异性IgM抗体及其持续性：与免疫荧光法和补体结合试验的比较
J Infect Dis. 1983 Sep;148(3):477-87. doi: 10.1093/infdis/148.3.477.
9
Immunoglobulin responses to Coxiella burnetii (Q fever): single-serum diagnosis of acute infection, using an immunofluorescence technique.针对伯氏考克斯体（Q热）的免疫球蛋白反应：采用免疫荧光技术对急性感染进行单份血清诊断。
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A microagglutination technique for detection and measurement of rickettsial antibodies.一种用于检测和测量立克次氏体抗体的微量凝集技术。
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安大略省奶山羊群中伯氏考克斯氏体感染的血清学调查。两种酶联免疫吸附测定方法的比较。

Serosurvey of Coxiella burnetii infection in dairy goat herds in Ontario. A comparison of two methods of enzyme-linked immunosorbent assay.

作者信息

Lang G H

机构信息

Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.

出版信息

Can J Vet Res. 1988 Jan;52(1):37-41.

PMID:3349400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1255397/
Abstract

Two technical variations of enzyme-linked immunosorbent assay for the detection of antibodies to Coxiella burnetii were compared in this serosurvey on 20 Ontario dairy goat herds. Both a trichloracetic acid extract and a coctoantigen of purified coxiellas were used to sensitize the microtitration plates. Technical differences related to coating pH, serum dilutions tested and interpretation of results. Results agreed in 98.6% of sera examined, the differing sera were in the low titer borderline range. Only 20% of the herds had seroreactors.

摘要

在对安大略省20个奶山羊群进行的血清学调查中，比较了用于检测伯氏考克斯氏体抗体的酶联免疫吸附测定的两种技术变体。三氯乙酸提取物和纯化考克斯氏体的煮沸抗原均用于使微量滴定板致敏。技术差异涉及包被pH值、检测的血清稀释度以及结果的解读。在检测的血清中，98.6%的结果一致，不同的血清处于低滴度临界范围。只有20%的羊群有血清反应阳性动物。