安大略省奶山羊群中伯氏考克斯氏体感染的血清学调查。两种酶联免疫吸附测定方法的比较。
Serosurvey of Coxiella burnetii infection in dairy goat herds in Ontario. A comparison of two methods of enzyme-linked immunosorbent assay.
作者信息
Lang G H
机构信息
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
出版信息
Can J Vet Res. 1988 Jan;52(1):37-41.
Abstract
Two technical variations of enzyme-linked immunosorbent assay for the detection of antibodies to Coxiella burnetii were compared in this serosurvey on 20 Ontario dairy goat herds. Both a trichloracetic acid extract and a coctoantigen of purified coxiellas were used to sensitize the microtitration plates. Technical differences related to coating pH, serum dilutions tested and interpretation of results. Results agreed in 98.6% of sera examined, the differing sera were in the low titer borderline range. Only 20% of the herds had seroreactors.
摘要
在对安大略省20个奶山羊群进行的血清学调查中，比较了用于检测伯氏考克斯氏体抗体的酶联免疫吸附测定的两种技术变体。三氯乙酸提取物和纯化考克斯氏体的煮沸抗原均用于使微量滴定板致敏。技术差异涉及包被pH值、检测的血清稀释度以及结果的解读。在检测的血清中，98.6%的结果一致，不同的血清处于低滴度临界范围。只有20%的羊群有血清反应阳性动物。
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