Simas Frauches Nayara, Montenegro Júlia, Amaral Thuane, Abreu Joel Pimentel, Laiber Gabriela, Junior Jorge, Borguini Renata, Santiago Manuela, Pacheco Sidney, Nakajima Vania Mayumi, Godoy Ronoel, Teodoro Anderson Junger
Laboratory of Functional Foods, Federal University of Rio de Janeiro State, Avenida Pasteur 296-Urca, 22290-240 Rio de Janeiro, RJ, Brazil.
Federal University Fluminense, Mario Santos Braga Street, 30-Centro, 24020-140 Niteroí, RJ, Brazil.
Molecules. 2021 Jan 22;26(3):564. doi: 10.3390/molecules26030564.
There is a significant indication of the beneficial health effects of fruit rich diets. Fruits of native plant species have noticeably different phytochemicals and bioactive effects. The aim of this work was to characterize and compare the constituents of jabuticaba (, MJ), jamun-berry (, SC), and malay-apple (, SM) extracts and their influence on antioxidant activity in vitro and antiproliferative effects on human colon adenocarcinoma cells. According to the results, dried peel powders (DP) have a high anthocyanin content, phenolic compounds, and antioxidant activity when compared to freeze dried extracts (FD). dried peel powder extract had a higher total anthocyanin and phenolic compounds content (802.90 ± 1.93 and 2152.92 ± 43.95 mg/100 g, respectively). A reduction in cell viability of HT-29 cells after treatment with extracts (DP-MJ and FD-MJ) was observed via MTT assay. Flow cytometry showed that the treatment with the anthocyanin-rich extracts from MJ, SC, and SM had an inhibitory impact on cell development due to G/M arrest and caused a rise in apoptotic cells in relation to the control group. The findings of this study highlight the potential of peel powders from fruits as an important source of natural antioxidants and a protective effect against colon adenocarcinoma.
有充分迹象表明富含水果的饮食对健康有益。本地植物物种的果实具有明显不同的植物化学物质和生物活性作用。本研究的目的是对巴西葡萄（, MJ）、印度乌墨（, SC）和马来苹果（, SM）提取物的成分进行表征和比较，并研究它们对体外抗氧化活性以及对人结肠腺癌细胞的抗增殖作用的影响。结果显示，与冷冻干燥提取物（FD）相比，干果皮粉（DP）具有较高的花青素含量、酚类化合物含量和抗氧化活性。 干果皮粉提取物的总花青素和酚类化合物含量更高（分别为802.90 ± 1.93和2152.92 ± 43.95 mg/100 g）。通过MTT法检测发现，用 提取物（DP-MJ和FD-MJ）处理后，HT-29细胞的活力降低。流式细胞术显示，用来自MJ、SC和SM的富含花青素的提取物处理会因G/M期阻滞而对细胞发育产生抑制作用，并导致相对于对照组凋亡细胞增加。本研究结果突出了 果实的果皮粉作为天然抗氧化剂的重要来源以及对结肠腺癌的保护作用的潜力。
