Universidade Federal de Alagoas (UFAL), Faculdade de Odontologia, Departamento de Odontologia Restauradora, Maceió, Brasil.
Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Preventiva e Restauradora, Araçatuba, Brasil.
J Appl Oral Sci. 2021 Jan 25;29:e20210483. doi: 10.1590/1678-7757-2020-0483. eCollection 2021.
The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats.
16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann-Whitney U test and Student's t test were performed (P<.05).
The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05).
Probiotic Complex modulates inflammation and bone resorption in apical periodontitis.
本研究旨在评估多菌株益生菌配方是否会影响大鼠根尖周炎（AP）的发展。
将 16 只 Wistar 大鼠分为两组（n=8）：常规饮食喂养的 AP 大鼠（对照-C（CG））；常规饮食喂养并补充多菌株配方（十亿个菌落形成单位（CFU））：GNC 益生菌复合物（PCG）（嗜酸乳杆菌、唾液乳杆菌、植物乳杆菌、鼠李糖乳杆菌、双歧杆菌双歧亚种、动物双歧杆菌亚种和嗜热链球菌）的 AP 大鼠（PCG）。通过牙髓暴露于口腔环境在上颌和下颌第一磨牙中诱导 AP。在 AP 发展期间，通过灌胃口服给予 PCG 30 天。在此期间后，处死动物，取出下颌骨并进行组织学分析和免疫化学检测白细胞介素（IL）-6、IL-10、IL-1β、RANKL、OPG 和 TRAP。进行 Mann-Whitney U 检验和 Student t 检验（P<.05）。
CG 显示比 PCG 组更强烈的炎症浸润（P<.05）。与 CG 相比，PCG 组的 IL-1β、IL 6 和 RANKL 降低（P<.05）。PCG 组的 IL-10 水平增加（P<.05）。两组的 OPG 水平相似（P>.05）。与 CG 相比，PCG 组的成熟破骨细胞（TRAP 阳性多核细胞）数量减少（P<.05）。
益生菌复合物可调节根尖周炎的炎症和骨吸收。
