The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, Fujian, China.
Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, Jiangsu, China.
Anal Bioanal Chem. 2021 Jul;413(18):4563-4579. doi: 10.1007/s00216-020-03124-3. Epub 2021 Jan 28.
Infectious diseases caused by pathogenic microorganisms such as viruses and bacteria pose a great threat to human health. Although a significant progress has been obtained in the diagnosis and prevention of infectious diseases, it still remains challenging to develop rapid and cost-effective detection approaches and overcome the side effects of therapeutic agents and pathogen resistance. Functional nucleic acids (FNAs), especially the most widely used aptamers and DNAzymes, hold the advantages of high stability and flexible design, which make them ideal molecular recognition tools for bacteria and viruses, as well as potential therapeutic drugs for infectious diseases. This review summarizes important advances in the selection and detection of bacterial- and virus-associated FNAs, along with their potential prevention ability of infectious disease in recent years. Finally, the challenges and future development directions are concluded.
由病毒和细菌等致病微生物引起的传染病对人类健康构成了巨大威胁。尽管在传染病的诊断和预防方面已经取得了重大进展，但开发快速、经济高效的检测方法并克服治疗剂的副作用和病原体耐药性仍然具有挑战性。功能核酸（FNAs），特别是应用最广泛的适体和 DNA 酶，具有稳定性高和设计灵活的优点，使其成为细菌和病毒的理想分子识别工具，以及治疗传染病的潜在治疗药物。本文综述了近年来在细菌和病毒相关 FNAs 的筛选和检测及其对传染病的潜在预防能力方面的重要进展。最后总结了挑战和未来的发展方向。
