Stoica G, O'Leary M
Texas A&M University, Department of Veterinary Pathology, College Station 77843.
J Cancer Res Clin Oncol. 1988;114(2):142-8. doi: 10.1007/BF00417828.
An N-ethyl-N-nitrosourea-induced rat ovarian Sertoli cell tumor was grown in tissue culture in Dulbecco's modified Eagle's medium (DMEM) supplemented with 25% horse serum (HS) and a hormone combination of 20 ng/ml each of hydrocortisone, insulin, and prolactin. This tissue culture derived from a nonsteroid hormone-producing tumor. Cytofluorometry and karyotyping of the nonhormone-producing tumor cell line (SCTL-1) revealed a diploid pattern for the early passage (P1), which became hyperdiploid (P10), and then aneuploid (P20). These cells had an epitheloid pattern, grew in a monolayer at early passages. After P10 the cells were transplanted into newborn rats and nude mice and resulted in high incidences of tumors (up to 100%). The cell line (SCTL-1) continued to grow in DMEM, 10% HS, and no hormone supplementation after P10. This study revealed that a benign rat ovarian Sertoli cell tumor after multiple passages in vitro underwent sequential genotypic and phenotypic changes and became highly malignant.
用N-乙基-N-亚硝基脲诱导产生的大鼠卵巢支持细胞瘤在含有25%马血清（HS）以及20 ng/ml氢化可的松、胰岛素和催乳素的激素组合的杜尔贝科改良伊格尔培养基（DMEM）中进行组织培养。这种组织培养物源自一种不产生类固醇激素的肿瘤。对不产生激素的肿瘤细胞系（SCTL-1）进行细胞荧光测定和核型分析发现，早期传代（P1）时呈现二倍体模式，传至第10代（P10）时变为超二倍体，然后在第20代（P20）时变为非整倍体。这些细胞具有上皮样形态，在早期传代时呈单层生长。P10之后，将这些细胞移植到新生大鼠和裸鼠体内，导致肿瘤高发（高达100%）。P10之后，细胞系（SCTL-1）在不含激素补充物的DMEM和10% HS中继续生长。这项研究表明，一种良性大鼠卵巢支持细胞瘤在体外多次传代后经历了一系列基因型和表型变化，变得高度恶性。
