College of Animal Science and Technology, Yangzhou University, No.48 of East Wenhui Road, Yangzhou, Jiangsu Province 225009, PR China.
College of Animal Science and Technology, Yangzhou University, No.48 of East Wenhui Road, Yangzhou, Jiangsu Province 225009, PR China.
Animal. 2021 Jan;15(1):100037. doi: 10.1016/j.animal.2020.100037. Epub 2020 Dec 9.
Meishan pigs tend to have higher disease resistance than commercial breeds, although more studies are needed to confirm this difference. This study compared intestinal barrier function between Meishan and crossbred neonatal piglets to provide guidance for both the breeding and nutritional regulation of pigs. Six Meishan piglets and 6 Duroc × (Landrace × Yorkshire) crossbred neonatal piglets (all with normal birth weights) were obtained and allocated into the MEIS and CROSS groups, respectively. Intestinal morphology, goblet cell numbers, antioxidant enzyme activity, and cytokine gene and tight junction protein expression were assessed. The results showed that BW was lower in the MEIS group than in the CROSS group (P < 0.01). The relative lengths of the duodenum (P < 0.05), jejunum (P < 0.01) and ileum (P < 0.01) in the MEIS group were higher than those in the CROSS group. Compared with the CROSS group, the MEIS group exhibited shorter villus lengths in the duodenum and jejunum (P < 0.01), a shallower crypt depth in the ileum (P < 0.001) and denser and longer microvilli in the intestine. The numbers of GCs in the duodenum (P < 0.01) and jejunum (P < 0.001) and the activity levels of glutathione peroxidase (P < 0.05) in the jejunum and of catalase (P < 0.01) and superoxide dismutase (P < 0.01) in the ileum were higher in the MEIS group than in the CROSS group. Compared with the CROSS group, the MEIS group exhibited higher gene expression levels of interleukin (IL) 4 and interferon γ (IFNγ) in the jejunum (P < 0.05); IL2 (P < 0.05), IL4 (P < 0.01) and IFNγ (P < 0.001) in the ileum; and mucin 2 (P < 0.01) and occludin (P < 0.05) in the duodenum. In conclusion, Meishan neonatal piglets showed lower birth weights but higher intestinal barrier function than crossbred piglets.
梅山猪比商业品种具有更高的抗病能力，尽管还需要更多的研究来证实这一差异。本研究比较了梅山猪和杂交仔猪新生仔猪的肠道屏障功能，为猪的饲养和营养调节提供指导。本研究共获得 6 头梅山猪和 6 头杜洛克×（长白猪×约克夏猪）杂交仔猪（均具有正常出生体重），并分别分配到 MEIS 和 CROSS 组。评估了肠道形态、杯状细胞数量、抗氧化酶活性以及细胞因子基因和紧密连接蛋白的表达。结果表明，MEIS 组的 BW 低于 CROSS 组（P<0.01）。MEIS 组十二指肠（P<0.05）、空肠（P<0.01）和回肠（P<0.01）的相对长度均高于 CROSS 组。与 CROSS 组相比，MEIS 组十二指肠和空肠的绒毛长度较短（P<0.01），回肠的隐窝深度较浅（P<0.001），肠黏膜绒毛更密集、更长。MEIS 组十二指肠（P<0.01）和空肠（P<0.001）的 GC 数量以及空肠的谷胱甘肽过氧化物酶活性（P<0.05）和回肠的过氧化氢酶活性（P<0.01）和超氧化物歧化酶活性（P<0.01）均高于 CROSS 组。与 CROSS 组相比，MEIS 组空肠白细胞介素（IL）4和干扰素（IFNγ）基因表达水平较高（P<0.05）；空肠 IL2（P<0.05）、IL4（P<0.01）和 IFNγ（P<0.001）；空肠黏蛋白 2（P<0.01）和 occludin（P<0.05）。综上所述，梅山仔猪出生体重较低，但肠道屏障功能高于杂交仔猪。
