Russo Isabella, Della Gatta Paul A, Garnham Andrew, Porter Judi, Burke Louise M, Costa Ricardo J S
Department of Nutrition Dietetics & Food, Monash University, Notting Hill, VIC, Australia.
School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition, Deakin University, Geelong, VIC, Australia.
Front Nutr. 2021 Jan 14;7:622270. doi: 10.3389/fnut.2020.622270. eCollection 2020.
This study aimed to determine the effects of flavored dairy milk based recovery beverages of different nutrition compositions on markers of gastrointestinal and immune status, and subsequent recovery optimisation markers. After completing 2 h high intensity interval running, participants ( = 9) consumed a whole food dairy milk recovery beverage (CM, 1.2 g/kg body mass (BM) carbohydrate and 0.4 g/kg BM protein) or a dairy milk based supplement beverage (MBSB, 2.2 g/kg BM carbohydrate and 0.8 g/kg BM protein) in a randomized crossover design. Venous blood samples, body mass, body water, and breath samples were collected, and gastrointestinal symptoms (GIS) were measured, pre- and post-exercise, and during recovery. Muscle biopsies were performed at 0 and 2 h of recovery. The following morning, participants returned to the laboratory to assess performance outcomes. In the recovery period, carbohydrate malabsorption (breath H peak: 49 . 24 ppm) occurred on MBSB compared to CM, with a trend toward greater gut discomfort. No difference in gastrointestinal integrity (i.e., I-FABP and sCD14) or immune response (i.e., circulating leukocyte trafficking, bacterially-stimulated neutrophil degranulation, and systemic inflammatory profile) markers were observed between CM and MBSB. Neither trial achieved a positive rate of muscle glycogen resynthesis [-25.8 (35.5) mmol/kg dw/h]. Both trials increased phosphorylation of intramuscular signaling proteins. Greater fluid retention (total body water: 86.9 . 81.9%) occurred on MBSB compared to CM. Performance outcomes did not differ between trials. The greater nutrient composition of MBSB induced greater gastrointestinal functional disturbance, did not prevent the post-exercise reduction in neutrophil function, and did not support greater overall acute recovery.
本研究旨在确定不同营养成分的调味乳制恢复饮料对胃肠道和免疫状态标志物以及后续恢复优化标志物的影响。在完成2小时的高强度间歇跑步后，参与者（n = 9）采用随机交叉设计饮用全食物乳制恢复饮料（CM，碳水化合物含量为1.2 g/kg体重（BM），蛋白质含量为0.4 g/kg BM）或乳制补充饮料（MBSB，碳水化合物含量为2.2 g/kg BM，蛋白质含量为0.8 g/kg BM）。在运动前、运动后和恢复期间采集静脉血样、体重、身体水分和呼吸样本，并测量胃肠道症状（GIS）。在恢复的0小时和2小时进行肌肉活检。第二天早上，参与者返回实验室评估运动表现结果。在恢复期间，与CM相比，MBSB出现了碳水化合物吸收不良（呼气H峰值：49.24 ppm），且肠道不适有加重趋势。在CM和MBSB之间未观察到胃肠道完整性（即I-FABP和sCD14）或免疫反应（即循环白细胞运输、细菌刺激的中性粒细胞脱颗粒和全身炎症谱）标志物的差异。两项试验均未达到肌肉糖原再合成的阳性率[-25.8（35.5）mmol/kg dw/h]。两项试验均增加了肌肉内信号蛋白的磷酸化。与CM相比，MBSB出现了更大的液体潴留（全身水分：86.9.81.9%）。试验之间运动表现结果没有差异。MBSB中更高的营养成分导致了更大的胃肠道功能紊乱，未能防止运动后中性粒细胞功能的降低，也不支持更好的整体急性恢复。
