Lyu Lina, Lai Qiliang, Li Jianyang, Yu Zhiqiang, Shao Zongze
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004685. Epub 2021 Feb 2.
Strain 11-3 was isolated from the surface seawater along the coast of Xiamen Island, China. Cells were Gram-stain-negative, oxidase- and catalase-positive, short and rod-shaped, nonmotile, 0.5-1.0 μm in width and 1.0-2.0 μm in length. Growth of strain 11-3 was at temperature of 15-37°C (optimum 28-35°C), at pH of 5.0-11.0 (optimum 7.0-9.0) and at salinity range of 0-10 (optimum 0.5-1). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 11-3 belonged to the genus and had the highest similarity with MJ17 (98.1 %), followed by 12-3 (97.1 %), ATCC 21588 (97.1 %), DSM 19484 (97.0 %), 2251 (97.0 %), KCTC 22803 (97.0 %) and other species of the genus (95.2-96.8 %). The DNA-DNA hybridization values between strain 11-3 and the selected strains ( MJ17, 12-3, ATCC 21588, DSM 19484 and 2251) were 19.4, 19.5, 21.6, 19.3 and 19.8 %, respectively. Corresponding, their ANI values were 77.53, 75.61, 75.36, 75.73 and 75.33 %, respectively. The major fatty acid was summed feature 8 (C 6/7). The major respiratory quinone was Q10. The polar lipids included phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), unidentified glycolipid (GL) and unidentified aminolipid (AL). The DNA G+C content of strain 11-3 was 60.1 %. Based on results of the phylogenetic analysis, phenotypic and chemotaxonomic characteristics, strain 11-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 11-3 (=MCCC 1A16380=KCTC 72689).