Lorigo Margarida, Quintaneiro Carla, Breitenfeld Luiza, Cairrao Elisa
CICS-UBI, Health Sciences Research Centre, University of Beira Interior, 6200-506 Covilhã, Portugal.
FCS-UBI, Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilhã, Portugal.
Biomedicines. 2021 Jan 26;9(2):115. doi: 10.3390/biomedicines9020115.
Increasing evidence relating the exposure and/or bioaccumulation of endocrine-disrupting compounds (EDCs) with cardiovascular system are arising. Octylmethoxycinnamate (OMC) is the most widely used UV-B filter and as EDC interacts with TH receptors. However, their effects on thyroid diseases during pregnancy remain unknown. The purpose of this work was to assess the short- and long-term effects of OMC on arterial tonus of pregnant women with hypothyroidism. To elucidate this, human umbilical artery (HUA) rings without endothelium were used to explore the vascular effects of OMC by arterial and cellular experiments. The binding energy and the modes of interaction of the OMC into the active center of the TSHR and THRα were analyzed by molecular docking studies. Our results indicated that OMC altered the contractility patterns of HUA contracted with serotonin, histamine and KCl, possibly due to an interference with serotonin and histamine receptors or an involvement of the Ca channels. The molecular docking analysis show that OMC compete with T for the binding center of THRα. Taken together, these findings pointed out to alterations in HUA reactivity as result of OMC-exposure, which may be involved in the development and increased risk of cardiovascular diseases.
越来越多的证据表明，内分泌干扰化合物（EDC）的暴露和/或生物累积与心血管系统有关。桂皮酸盐（OMC）是使用最广泛的UV-B滤光剂，作为EDC可与甲状腺激素（TH）受体相互作用。然而，其对孕期甲状腺疾病的影响尚不清楚。这项工作的目的是评估OMC对甲状腺功能减退孕妇动脉张力的短期和长期影响。为阐明这一点，采用无内皮的人脐动脉（HUA）环，通过动脉和细胞实验探索OMC的血管效应。通过分子对接研究分析了OMC与促甲状腺激素受体（TSHR）和甲状腺激素受体α（THRα）活性中心的结合能及相互作用模式。我们的结果表明，OMC改变了由血清素、组胺和氯化钾收缩的HUA的收缩模式，这可能是由于对血清素和组胺受体的干扰或钙通道的参与。分子对接分析表明，OMC与甲状腺激素（T）竞争THRα的结合中心。综上所述，这些发现指出，OMC暴露导致HUA反应性改变，这可能与心血管疾病的发生和风险增加有关。
