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MYCN 扩增神经母细胞瘤细胞衍生的外泌体 miR-17-5p 促进非 MYCN 扩增细胞的增殖和迁移。

MYCN‑amplified neuroblastoma cell‑derived exosomal miR‑17‑5p promotes proliferation and migration of non‑MYCN amplified cells.

机构信息

Department of Pediatric Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

Department of Biochemistry and Molecular Biology of Basic Medical College, Qingdao University, Qingdao, Shandong 266071, P.R. China.

出版信息

Mol Med Rep. 2021 Apr;23(4). doi: 10.3892/mmr.2021.11884. Epub 2021 Feb 4.

DOI:10.3892/mmr.2021.11884
PMID:33537818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7893779/
Abstract

Neuroblastoma (NB) is considered a highly prevalent extracranial solid tumor in young children, and the upregulation of N‑myc proto‑oncogene (MYCN) is closely associated with the late stages of NB and poor prognostic outcomes. The current study was designed to evaluate the effects of exosomal microRNA (miRNA/miR)‑17‑5p from MYCN‑amplified NB cells on the proliferative and migratory potential of non‑MYCN amplified NB cells. miR‑17‑5p was found to activate the PI3K/Akt signaling cascade by targeting PTEN, and the overexpression of miR‑17‑5p was found to promote cellular migration and proliferation . Further experimentation revealed that the elevated expression of miR‑17‑5p in SK‑N‑BE(2) cell‑derived exosomes significantly promoted the proliferative and migratory capacities of SH‑SY5Y cells by inhibiting PTEN. Collectively, these findings demonstrated that miR‑17‑5p derived from MYCN‑amplified NB cell exosomes promoted the migration and proliferation of non‑MYCN amplified cells, highlighting an exosome‑associated malignant role for miR‑17‑5p.

摘要

神经母细胞瘤（NB）被认为是儿童中一种高发的颅外实体瘤，N- myc 原癌基因（MYCN）的上调与 NB 的晚期和不良预后密切相关。本研究旨在评估来自 MYCN 扩增 NB 细胞的外泌体 microRNA（miRNA/miR）-17-5p 对非 MYCN 扩增 NB 细胞增殖和迁移能力的影响。miR-17-5p 通过靶向 PTEN 激活 PI3K/Akt 信号级联，过表达 miR-17-5p 可促进细胞迁移和增殖。进一步的实验表明，SK-N-BE（2）细胞衍生的外泌体中 miR-17-5p 的高表达通过抑制 PTEN 显著促进了 SH-SY5Y 细胞的增殖和迁移能力。总之，这些发现表明，来自 MYCN 扩增 NB 细胞外泌体的 miR-17-5p 促进了非 MYCN 扩增细胞的迁移和增殖，突出了 miR-17-5p 在外泌体相关恶性作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/c089d2b51ea8/mmr-23-04-11884-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/dcc9533bcb6a/mmr-23-04-11884-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/0f909a2f84c8/mmr-23-04-11884-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/df7267e71ed5/mmr-23-04-11884-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/c089d2b51ea8/mmr-23-04-11884-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/dcc9533bcb6a/mmr-23-04-11884-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/0f909a2f84c8/mmr-23-04-11884-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/df7267e71ed5/mmr-23-04-11884-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d214/7893779/c089d2b51ea8/mmr-23-04-11884-g03.jpg

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