Grubman M J, Mebus C, Dale B, Yamanaka M, Yilma T
U.S. Department of Agriculture, ARS, NAA, Plum Island Animal Disease Center, Greenport, New York 11944.
Virology. 1988 Apr;163(2):261-7. doi: 10.1016/0042-6822(88)90265-6.
We have identified, by [35S]methionine labeling, eight major induced proteins and a number of minor proteins in rinderpest virus-infected bovine kidney cells. The polypeptides ranged in molecular weight from 212 to 21.5 kDa. The majority of these polypeptides are virus specific, as demonstrated by immunoprecipitation with rabbit hyperimmune serum against rinderpest. Infected cells radiolabeled with glucosamine contained a 75-kDa polypeptide and a broad band migrating at 80 kDa, both identified as virus specific by immunoprecipitation. Phosphorylated virus-specific proteins of 65 kDa and a complex of polypeptides at 92.5 kDa were also identified. Monospecific and monoclonal antibodies against measles virus and canine distemper virus hemagglutinin, fusion protein, nucleocapsid protein, and phosphoproteins confirmed the identity of the corresponding rinderpest virus-specific polypeptides.
通过[35S]甲硫氨酸标记，我们在感染牛瘟病毒的牛肾细胞中鉴定出了8种主要的诱导蛋白和一些次要蛋白。这些多肽的分子量范围为212至21.5 kDa。通过用抗牛瘟的兔超免疫血清进行免疫沉淀证明，这些多肽中的大多数是病毒特异性的。用葡糖胺进行放射性标记的感染细胞含有一种75 kDa的多肽和一条在80 kDa处迁移的宽带，通过免疫沉淀均鉴定为病毒特异性的。还鉴定出了65 kDa的磷酸化病毒特异性蛋白和92.5 kDa的多肽复合物。针对麻疹病毒和犬瘟热病毒血凝素、融合蛋白、核衣壳蛋白和磷蛋白的单特异性和单克隆抗体证实了相应牛瘟病毒特异性多肽的身份。
