Department of Physical Medicine, Rehabilitation and Occupational Therapy, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Wien Klin Wochenschr. 2022 Jan;134(1-2):39-44. doi: 10.1007/s00508-021-01815-0. Epub 2021 Feb 5.
Circulating serum sclerostin levels are supposed to give a good estimation of the levels of this negative regulator of bone mass within bone. Most studies evaluating total serum sclerostin found different levels in males compared to females and in older compared to younger subjects. Besides an ELISA detecting total sclerostin an ELISA determining bioactive sclerostin has been developed. The aim of this study was to investigate serum levels of bioactive sclerostin in an Austrian population-based cohort.
We conducted a cross-sectional observational study in 235 healthy subjects. Using the bioactive ELISA assay (Biomedica) bioactive sclerostin levels were evaluated.
Serum levels of bioactive sclerostin were higher in men than in women (24%). The levels correlated positively with age (r = 0.47). A positive correlation could also be detected with body mass index and bone mineral density.
Using the ELISA detecting bioactive sclerostin our results are consistent with data in the literature obtained by different sclerostin assays. The determination of sclerostin concentrations in peripheral blood thus appears to be a robust parameter of bone metabolism.
循环血清中骨硬化蛋白水平可很好地估计其在骨内对骨量的负调控水平。大多数评估总血清骨硬化蛋白的研究发现，男性与女性、老年与年轻受试者之间的水平存在差异。除了检测总骨硬化蛋白的 ELISA 外，还开发了一种检测生物活性骨硬化蛋白的 ELISA。本研究旨在调查奥地利基于人群的队列中生物活性骨硬化蛋白的血清水平。
我们对 235 名健康受试者进行了横断面观察性研究。使用生物活性 ELISA 测定法（Biomedica）评估生物活性骨硬化蛋白水平。
血清生物活性骨硬化蛋白水平男性高于女性（24%）。水平与年龄呈正相关（r=0.47）。还可以检测到与体重指数和骨密度的正相关。
使用检测生物活性骨硬化蛋白的 ELISA，我们的结果与使用不同骨硬化蛋白测定法获得的文献数据一致。因此，外周血中骨硬化蛋白浓度的测定似乎是骨代谢的一个可靠参数。
