Liu J, Wang X E, Lv D, Qiao M, Zhang L, Meng H X, Xu L, Mao M X
Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Dec 9;53(1):16-23. doi: 10.19723/j.issn.1671-167X.2021.01.004.
To explore the association between the abnormal root morphology and bone metabolism or root development related gene polymorphism in patients with generalized aggressive periodontitis.
In the study, 179 patients with generalized aggressive periodontitis were enrolled, with an average age of (27.23±5.19) years, male / female = 67/112. The average number of teeth remaining in the mouth was (26.80±1.84). Thirteen single nucleotide polymorphisms (SNPs) of nine genes which related to bone metabolism and root development were detected by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Root abnormalities were identified using periapical radiographs. The abnormal root morphology included cone-rooted teeth, slender-root teeth, short-rooted teeth, curved-rooted teeth, syncretic-rooted molars, and molar root abnormalities. The number of teeth and incidence of abnormal root morphology in different genotypes of 13 SNPs were analyzed.
The constituent ratio of root with root abnormality in GAgP patients was 14.49%(695/4 798). The average number of teeth with abnormal root morphology in GAgP was (3.88±3.84). The average number of teeth with abnormal root morphology in CC, CT and TT genotypes in vitamin D receptor (VDR) rs2228570 was (4.66±4.10), (3.71±3.93) and (2.68±2.68). There was significant difference between TT genotype and CC genotype ( = 2.62, =0.01). The average number of root morphological abnormalities in CC, CT and TT genotypes of Calcitotin Receptor (CTR) gene rs2283002 was (5.02±3.70), (3.43±3.95), and (3.05±3.12). The incidence of root morphological abnormalities in CC genotype was higher than that in the patients with CT and TT, and the difference was statistically significant(87.86% . 65.26% & 63.64%, =0.006, adjusted =3.71, 95%: 1.45-9.50). There was no significant difference in the incidence of abnormal root morphology between CT and TT genotypes.
VDR rs2228570 and CTR rs2283002 may be associated with the occurrence of abnormal root morphology in patients with generalized aggressive periodontitis, which is worthy of further research.
探讨广泛型侵袭性牙周炎患者牙根形态异常与骨代谢或牙根发育相关基因多态性之间的关联。
本研究纳入179例广泛型侵袭性牙周炎患者，平均年龄为（27.23±5.19）岁，男/女 = 67/112。口腔内余留牙平均数量为（26.80±1.84）颗。采用基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）检测9个与骨代谢和牙根发育相关基因的13个单核苷酸多态性（SNP）。通过根尖片确定牙根异常情况。异常牙根形态包括锥形根牙、细长根牙、短根牙、弯曲根牙、融合根磨牙以及磨牙牙根异常。分析13个SNP不同基因型的牙齿数量及牙根形态异常发生率。
广泛型侵袭性牙周炎患者牙根异常的构成比为14.49%（695/4798）。广泛型侵袭性牙周炎患者牙根形态异常的牙齿平均数量为（3.88±3.84）颗。维生素D受体（VDR）rs2228570基因CC、CT和TT基因型中牙根形态异常的牙齿平均数量分别为（4.66±4.10）颗、（3.71±3.93）颗和（2.68±2.68）颗。TT基因型与CC基因型之间存在显著差异（ = 2.62， =0.01）。降钙素受体（CTR）基因rs2283002的CC、CT和TT基因型中牙根形态异常的平均数量分别为（5.02±3.70）颗、（3.43±3.95）颗和（3.05±3.12）颗。CC基因型牙根形态异常的发生率高于CT和TT基因型患者，差异具有统计学意义（87.86% 对65.26% & 63.64%， =0.006，校正后 =3.71，95%可信区间：1.45 - 9.50）。CT和TT基因型之间牙根形态异常发生率无显著差异。
VDR rs2228570和CTR rs2283002可能与广泛型侵袭性牙周炎患者牙根形态异常的发生有关，值得进一步研究。
