Azimkhanova Balzhan B, Ustenova Gulbaram O, Sharipov Kamalidin O, Rakhimov Kayrolla D, Sayakova Galiya M, Jumagaziyeva Ardak B, Flisyuk Elena V, Gemejiyeva Nadezhda G
Department of Pharmaceutical Technology, Asfendiyarov Kazakh National Medical University, Almaty 050000, Kazakhstan.
Department of Biochemistry, Asfendiyarov Kazakh National Medical University, Almaty 050000, Kazakhstan.
Int J Biomater. 2021 Aug 4;2021:4389967. doi: 10.1155/2021/4389967. eCollection 2021.
The genus L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties ( Waldst. et Kit., L., L., and L.), used in folk medicine as means of antibacterial, irritant, laxative, antitumor, analgesic, and anthelmintic action. . Raw materials were collected from Almaty region (Republic of Kazakhstan). herb's carbon dioxide extract (CO extract) was obtained by subcritical carbon dioxide extraction. A gas chromatograph with a mass spectrometric detector was used to determine the component composition of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilution and the disc-diffusion method. Four microbial test strains were used: ATCC 6538-P, ATCC 8739, ATCC 10031, and ATCC 10231. . The technology of carbon dioxide extraction has undoubted advantages over traditional methods of extraction: it has a controlled selectivity in relation to groups of biologically active substances, allows deep extraction, and maximizes the release of rich complexes of compounds contained in plants. In this study, firstly, the CO extract was obtained under subcritical conditions from the aerial part of L., and the composition was determined. Hexane was the best solvent for CO extract, and 40 components were identified. Screening of antimicrobial activity of the 's CO extract showed the essential activity of all clinically significant strains tested: , , , and . . This research showed that the CO extract of the raw material of L. contains biologically active compounds exhibiting an essential antimicrobial effect, and therefore it is possible to recommend for the development of various drugs for use in medical practice.
十字花科伯内特属植物分布几乎遍及全球，有150多个物种。在哈萨克斯坦，已描述了21个物种，其中4个具有药用特性（瓦尔德施泰特和基特、……、……、……），在民间医学中用作抗菌、刺激、泻药、抗肿瘤、止痛和驱虫药物。原材料采自哈萨克斯坦共和国阿拉木图地区。……植物的二氧化碳提取物（CO提取物）通过亚临界二氧化碳萃取获得。使用带有质谱检测器的气相色谱仪测定提取物的成分组成。通过两种方法测定抗菌活性：连续稀释微量法和纸片扩散法。使用了四种微生物测试菌株：美国典型培养物保藏中心6538 - P、美国典型培养物保藏中心8739、美国典型培养物保藏中心10031和美国典型培养物保藏中心10231。……与传统提取方法相比，二氧化碳提取技术具有明显优势：它对生物活性物质组具有可控的选择性，能够进行深度提取，并使植物中所含化合物的丰富复合物最大限度地释放出来。在本研究中，首先在亚临界条件下从……植物的地上部分获得CO提取物，并确定其成分。己烷是CO提取物的最佳溶剂，鉴定出40种成分。对……植物的CO提取物的抗菌活性筛选显示，对所有测试的临床重要菌株：……、……、……和……均有显著活性。……这项研究表明，……植物原材料的CO提取物含有具有显著抗菌作用的生物活性化合物，因此可推荐用于开发各种医疗实践中使用的药物。
