Boštjančić Ljudevit Luka, Bonassin Lena, Anušić Lucija, Lovrenčić Leona, Besendorfer Višnja, Maguire Ivana, Grandjean Frederic, Austin Christopher M, Greve Carola, Hamadou Alexander Ben, Mlinarec Jelena
Division of Molecular Biology, Department of Biology, University of Zagreb, Zagreb, Croatia.
Division of Zoology, Department of Biology, University of Zagreb, Zagreb, Croatia.
Front Genet. 2021 Jan 21;11:611745. doi: 10.3389/fgene.2020.611745. eCollection 2020.
is a native European crayfish species found in both freshwater and brackish environments. It has commercial importance for fisheries and aquaculture industries. Up till now, most studies concerning have focused onto gaining knowledge about its phylogeny and population genetics. However, little is known about the chromosomal evolution and genome organization of this species. Therefore, we performed clustering analysis of a low coverage genomic dataset to identify and characterize repetitive DNA in the genome. In addition, the karyogram of (2 = 180) is presented here for the first time consisting of 75 metacentric, 14 submetacentric, and a submetacentric/metacentric heteromorphic chromosome pair. We determined the genome size to be at 18.7 gigabase pairs. Repetitive DNA represents about 54.85% of the genome. Satellite DNA repeats are the most abundant type of repetitive DNA, making up to ~28% of the total amount of repetitive elements, followed by the Ty3/ retroelements (15%). Our study established a surprisingly high diversity of satellite repeats in . The genome of is by far the most satellite-rich genome discovered to date with 258 satellite families described. Of the five mapped satellite DNA families on chromosomes, PlSAT3-411 co-localizes with the AT-rich DAPI positive probable (peri)centromeric heterochromatin on all chromosomes, while PlSAT14-79 co-localizes with the AT-rich DAPI positive (peri)centromeric heterochromatin on one chromosome and is also located subterminally and intercalary on some chromosomes. PlSAT1-21 is located intercalary in the vicinity of the (peri)centromeric heterochromatin on some chromosomes, while PlSAT6-70 and PlSAT7-134 are located intercalary on some chromosomes. The FISH results reveal amplification of interstitial telomeric repeats (ITRs) in . The prevalence of repetitive elements, especially the satellite DNA repeats, may have provided a driving force for the evolution of the genome.
是一种原产于欧洲的小龙虾物种，在淡水和微咸水环境中均有发现。它对渔业和水产养殖业具有商业重要性。到目前为止，大多数关于它的研究都集中在了解其系统发育和种群遗传学方面。然而，关于该物种的染色体进化和基因组组织却知之甚少。因此，我们对低覆盖度基因组数据集进行了聚类分析，以识别和表征该物种基因组中的重复DNA。此外，首次展示了该物种（2n = 180）的核型图，其由75条中着丝粒染色体、14条亚中着丝粒染色体和一对亚中着丝粒/中着丝粒异型染色体组成。我们确定基因组大小约为18.7吉碱基对。重复DNA约占基因组的54.85%。卫星DNA重复序列是最丰富的重复DNA类型，占重复元件总量的约28%，其次是Ty3/反转录元件（约15%）。我们的研究在该物种中发现了惊人的卫星重复序列多样性。该物种的基因组是迄今为止发现的卫星序列最丰富的基因组，已描述了258个卫星家族。在染色体上定位的五个卫星DNA家族中，PlSAT3 - 411与所有染色体上富含AT的DAPI阳性可能的（近）着丝粒异染色质共定位，而PlSAT14 - 79与一条染色体上富含AT的DAPI阳性（近）着丝粒异染色质共定位，并且在一些染色体上也位于亚末端和中间位置。PlSAT1 - 21在一些染色体上位于（近）着丝粒异染色质附近的中间位置，而PlSAT6 - 70和PlSAT7 - 则位于一些染色体的中间位置。荧光原位杂交结果显示该物种存在间质端粒重复序列（ITRs）的扩增。重复元件的普遍存在，尤其是卫星DNA重复序列，可能为该物种基因组的进化提供了驱动力。
Zotero 插件
