Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China.
Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, China.
Environ Sci Technol. 2021 Apr 6;55(7):4115-4122. doi: 10.1021/acs.est.0c06962. Epub 2021 Feb 10.
The frequent detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in healthcare environments, accommodations, and wastewater has attracted great attention to the risk of viral transmission by environmental fomites. However, the process of SARS-CoV-2 adsorption to exposed surfaces in high-risk environments remains unclear. In this study, we investigated the interfacial dynamics of single SARS-CoV-2 pseudoviruses with plasmonic imaging technology. Through the use of this technique, which has high spatial and temporal resolution, we tracked the collision of viruses at a surface and differentiated their stable adsorption and transient adsorption. We determined the effect of the electrostatic force on virus adhesion by correlating the solution and surface chemistry with the interfacial diffusion velocity and equilibrium position. Viral adsorption was found to be enhanced in real scenarios, such as in simulated saliva. This work not only describes a plasmonic imaging method to examine the interfacial dynamics of a single virus but also provides direct measurements of the factors that regulate the interfacial adsorption of SARS-CoV-2 pseudovirus. Such information is valuable for understanding virus transport and environmental transmission and even for designing anticontamination surfaces.
在医疗环境、住宿和废水中频繁检测到严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）RNA，这引起了人们对环境污染物传播病毒风险的极大关注。然而，SARS-CoV-2 在高风险环境中暴露表面的吸附过程仍不清楚。在这项研究中，我们使用等离子体成像技术研究了单个人冠状病毒 2 假病毒与表面的界面动力学。通过使用这项具有高时空分辨率的技术，我们跟踪了病毒在表面的碰撞，并区分了它们的稳定吸附和瞬时吸附。我们通过将溶液和表面化学与界面扩散速度和平衡位置相关联，确定了静电力对病毒附着的影响。在模拟唾液等实际情况下，发现病毒吸附增强。这项工作不仅描述了一种等离子体成像方法来检查单个病毒的界面动力学，还提供了直接测量调节 SARS-CoV-2 假病毒界面吸附的因素。这些信息对于理解病毒的传播和环境传播很有价值，甚至对于设计防污染表面也很有价值。
