Yu Fei-Xue, Li Zhe, Chen Yao, Yang Yin-He, Li Guo-Hong, Zhao Pei-Ji
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming 650091, China; Faculty of medicine, Kunming University of Science and Technology, Kunming 650500, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Fitoterapia. 2017 Mar;117:41-46. doi: 10.1016/j.fitote.2016.12.012. Epub 2016 Dec 29.
An endophytic fungus, Chaetomium sp. M453, was isolated from Huperzia serrata (Thunb. ex Murray) Trev. and subjected to phytochemical investigation. Three unusual C25 steroids, neocyclocitrinols E-G (1-3), and 3β-hydroxy-5,9-epoxy-(22E,24R)-ergosta-7,22-dien-6-one (4) together with three known steroids were isolated from solid fermentation products of the fungus, which were elucidated by extensive spectroscopic analyses, including 1D-, 2D-NMR, and HR-ESI-MS experiments. The absolute configuration of 1 was determined by X-ray crystallographic analysis and CD analyses. The acetylcholinesterase inhibitory activities of compounds 1-4 were tested in vitro. Compound 4 showed weak acetylcholinesterase inhibitory activity.
从蛇足石杉中分离出一种内生真菌毛壳菌属M453，并对其进行了植物化学研究。从该真菌的固体发酵产物中分离出三种不寻常的C25甾体化合物，新环柠檬醇E-G（1-3）和3β-羟基-5,9-环氧-（22E，24R）-麦角甾-7,22-二烯-6-酮（4）以及三种已知的甾体化合物，通过广泛的光谱分析对其进行了鉴定，包括一维、二维核磁共振和高分辨电喷雾电离质谱实验。通过X射线晶体学分析和圆二色性分析确定了1的绝对构型。在体外测试了化合物1-4的乙酰胆碱酯酶抑制活性。化合物4显示出较弱的乙酰胆碱酯酶抑制活性。
