Wang Jiali, Wang Tiangong, Hu Shiyun, Li Jinyun, Ni Chao, Ye Meng
Department of Oncology and Hematology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, People's Republic of China.
School of Medicine, Ningbo University, Ningbo, People's Republic of China.
Cancer Manag Res. 2021 Feb 3;13:965-975. doi: 10.2147/CMAR.S289775. eCollection 2021.
Circular RNAs are novel endogenous RNAs, which are considered to play a role in tumorigenesis. Nevertheless, the role as well as clinical diagnostic value of most circular RNAs in colorectal cancer are still unclear.
We investigated the circular RNA microarray containing expression profiles in samples of colorectal cancer patients by bioinformatics. The consequence indicated that hsa_circ_0043278 was strongly downregulated. We then measured the expression level of hsa_circ_0043278 in tissue samples of colorectal cancer by quantitative real-time polymerase chain reaction. Besides, we also explored the expression condition of the circular RNA in colorectal cancer cell lines including HCT116, SW620, and SW480. Cell counting kit-8, colony formation, and transwell assays, as well as flow cytometry, were applied to detect changes in cell proliferation, migration, apoptosis, and cell cycle progression.
We discovered that circular RNA hsa_circ_0043278 was significantly downregulated in tumor samples ( < 0.0001) as well as cell lines ( < 0.05). The value of the area under the receiver operating characteristic curve was 0.71, with a sensitivity of 0.72 and specificity of 0.70 ( = 0.0006). Moreover, we found that overexpression of hsa_circ_0043278 suppressed proliferation and migratory abilities while promoting apoptosis in colorectal cancer cells.
Our findings revealed that hsa_circ_0043278 inhibited the tumorigenesis of colorectal cancer and could be a potential biomarker for colorectal cancer diagnosis. Besides, it hopes to become a target for treatment.
环状RNA是一类新型内源性RNA，被认为在肿瘤发生过程中发挥作用。然而，大多数环状RNA在结直肠癌中的作用及临床诊断价值仍不清楚。
我们通过生物信息学研究了包含结直肠癌患者样本表达谱的环状RNA微阵列。结果表明hsa_circ_0043278表达显著下调。然后我们通过定量实时聚合酶链反应测量了结直肠癌组织样本中hsa_circ_0043278的表达水平。此外，我们还探究了该环状RNA在包括HCT116、SW620和SW480在内的结直肠癌细胞系中的表达情况。应用细胞计数试剂盒-8、集落形成、transwell实验以及流式细胞术来检测细胞增殖、迁移、凋亡和细胞周期进程的变化。
我们发现环状RNA hsa_circ_0043278在肿瘤样本（<0.0001）以及细胞系中（<0.05）均显著下调。受试者工作特征曲线下面积值为0.71，灵敏度为0.72，特异性为0.70（=0.0006）。此外，我们发现hsa_circ_0043278的过表达抑制了结直肠癌细胞的增殖和迁移能力，同时促进其凋亡。
我们的研究结果表明hsa_circ_0043278抑制了结直肠癌的肿瘤发生，可能是结直肠癌诊断的潜在生物标志物。此外，有望成为治疗靶点。
