RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei-shi, Tokyo 184-8588, Japan.
J Agric Food Chem. 2021 Aug 18;69(32):8981-8990. doi: 10.1021/acs.jafc.0c07356. Epub 2021 Feb 11.
Lipids exhibit functional bioactivities based on their polar and acyl chain properties; humans obtain lipids from dietary plant product intake. Therefore, the identification of different molecular species facilitates the evaluation of biological functions and nutrition levels and new phenotype-modulating lipid structures. As a rapid screening strategy, we performed untargeted lipidomics for 155 agricultural products in 58 species from 23 plant families, wherein product-specific lipid diversities were shown using computational mass spectrometry. We characterized 716 lipid species, for which the profiles revealed the National Center for Biotechnology Information-established organismal classification and unique plant tissue metabotypes. Moreover, we annotated unreported subclasses in plant lipidology; e.g., triacylglycerol estolide (TG-EST) was detected in rice seeds () and several plant species. TG-EST is known as the precursor molecule producing the fatty acid ester of hydroxy fatty acid, which lowers ambient glycemia and improves glucose tolerance. Hence, our method can identify agricultural plant products containing valuable lipid ingredients.
脂质根据其极性和酰基链性质表现出功能生物活性；人类从食用植物产品中获取脂质。因此，不同分子种类的鉴定有助于评估生物功能和营养水平以及新的表型调节脂质结构。作为一种快速筛选策略，我们对来自 23 个植物科的 58 个物种的 155 种农产品进行了非靶向脂质组学分析，其中使用计算质谱显示了特定于产品的脂质多样性。我们鉴定了 716 种脂质，其图谱揭示了国家生物技术信息中心建立的生物体分类和独特的植物组织代谢类型。此外，我们还注释了植物脂质学中未报道的亚类；例如，在水稻种子（）和几种植物中检测到三酰基甘油酯（TG-EST）。TG-EST 被称为产生羟基脂肪酸脂肪酸酯的前体分子，可降低环境血糖并改善葡萄糖耐量。因此，我们的方法可以识别含有有价值脂质成分的农业植物产品。
