Zhong Yicheng, Zhao Yunlei, Wang Yue, Niu Juan, Sun Zhimin, Chen Jianhua, Luan Mingbao
Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Biology (Basel). 2022 Jun 3;11(6):855. doi: 10.3390/biology11060855.
(Thunb.) Koidz is an important Chinese medicinal and economic crop. Its seeds, which are rich in fatty acids, are usually discarded. As of now, lipid biosynthesis pathways and genes have not been clearly described. In this work, we found that seed and fruit development of were not synchronized, and that when the fruit was ripe, seed oil content was not at its highest. As seeds developed, linoleic and oleic acid content was found to decrease and increase, respectively. RNA sequencing yielded 108.45 GB of clean reads from 15 cDNA libraries, containing 8756 differentially expressed genes. We identified 65 unigenes associated with lipid biosynthesis, including fatty acid and triacylglycerol biosynthesis. The 65 unigenes were mapped to the lipid synthesis pathway. There were 20 family members in , which could be divided into four sub-groups with the highest number of . Our study revealed the dynamic changes in seed oil content and composition during its growth period and provides large-scale and comprehensive transcriptome data of seeds. These findings provide a basis for the improvement of seed oil yield and quality.
（拇指）小田原是一种重要的中药材和经济作物。其富含脂肪酸的种子通常被丢弃。截至目前，脂质生物合成途径和基因尚未得到清晰描述。在这项研究中，我们发现其种子和果实发育不同步，果实成熟时种子油含量并非最高。随着种子发育，亚油酸和油酸含量分别呈下降和上升趋势。RNA测序从15个cDNA文库中获得了108.45GB的 clean reads，包含8756个差异表达基因。我们鉴定出65个与脂质生物合成相关的单基因，包括脂肪酸和三酰甘油生物合成。这65个单基因被映射到脂质合成途径。小田原中有20个家族成员，可分为四个亚组，其中数量最多。我们的研究揭示了小田原种子油含量和成分在其生长期间的动态变化，并提供了小田原种子大规模且全面的转录组数据。这些发现为提高小田原种子油产量和质量提供了依据。
需要注意的是，原文中存在一些表述不完整或不太准确的地方，比如“(Thunb.) Koidz”不太明确具体所指，翻译可能会存在一定局限性。
