Institute of Entomology, Guizhou University, Guiyang 550025, China.
The Provincial Special Key Laboratory for Development and Utilization of Insect Resources, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2021 Jan 29;22(3):1348. doi: 10.3390/ijms22031348.
To explore the differences in mitogenome variation and phylogenetics among lineages of the Hemiptera superfamily Fulgoroidea, we sequenced four new mitogenomes of Caliscelidae: two species of the genus (Caliscelinae: Caliscelini), namely and , and two species of the genus (Ommatidiotinae: Augilini), namely and . The four mitogenomes were 15,922-16,640 bp (base pair) in length, with 37 mitochondrial genes and an AT-rich region. Gene content and arrangement were similar to those of most other sequenced hexapod mitogenomes. All protein-coding genes (PCGs) started with a canonical ATN or GTG and ended with TAA or an incomplete stop codon single T. Except for two transfer RNAs (tRNAs; trnS1 and trnV) lacking a dihydrouridine arm in the four species and trnC lacking a dihydrouridine stem in the species, the remaining tRNAs could fold into canonical cloverleaf secondary structures. Phylogenetic analyses based on sequence data of 13 PCGs in the 28 Fulgoroidea species and two outgroups revealed that Delphacidae was monophyletic with strong support. Our data suggest that Fulgoridae is more ancient than Achilidae. Furthermore, Flatidae, Issidae, and Ricaniidae always cluster to form a sister group to Caliscelidae.
为了探究半翅目飞虱总科中不同谱系的线粒体基因组变异和系统发育，我们对叶蝉科的 4 个新线粒体基因组进行了测序：属（叶蝉亚科：叶蝉族）的 2 个种，即 和 ，以及属（沫蝉亚科：澳沫蝉族）的 2 个种，即 和 。这 4 个线粒体基因组的长度分别为 15922-16640bp（碱基对），包含 37 个线粒体基因和一个富含 AT 的区域。基因的组成和排列与大多数已测序的六足动物线粒体基因组相似。所有蛋白质编码基因（PCGs）都以典型的 ATN 或 GTG 起始，以 TAA 或不完全终止密码子单个 T 结束。除了 4 个物种中的 2 个转移 RNA（trnS1 和 trnV）缺少二氢尿嘧啶臂以及 物种中的 trnC 缺少二氢尿嘧啶茎之外，其余的 tRNA 都能折叠成典型的三叶草二级结构。基于 28 种飞虱总科物种和 2 个外群的 13 个 PCGs 的序列数据进行的系统发育分析表明，角蝉科是单系的，具有很强的支持。我们的数据表明，蝉科比沫蝉科更为古老。此外，扁头蝉科、沫蝉科、硕蝉科和泥蝉科总是聚类形成与叶蝉科的姐妹群。
